Grace's Insect Medium, unsupplemented

L-グルタミンは含まれていますが、L-メチオニンや昆虫血リンパは含まれていません。昆虫細胞培養試験済み。詳細を見る

製品番号（カタログ番号）	数量
11595030	500 mL

製品番号（カタログ番号） 11595030

価格（JPY）

13,100

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500 mL

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L-グルタミンは含まれていますが、L-メチオニンや昆虫血リンパは含まれていません。

昆虫細胞培養試験済み。

研究用途にのみご使用ください。診断目的には使用できません。

細胞株Sf21, Sf9

細胞タイプInsect Cell

無機塩類Calcium, Magnesium

製品ラインGibco

製品タイプGrace's Insect Medium

数量500 mL

出荷条件Room Temperature

形状Liquid

添加剤ありGlutamine, Sodium Bicarbonate

添加剤なしNo Lactalbumin Hydrolysate, No Yeastolate, No Methionine

Unit SizeEach

組成および保存条件

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

For how long can I store Grace's Insect Medium, unsupplemented at room temperature?

We ship this product under “ambient” conditions throughout the year. We have performed shipping studies that shown components to be stable for at least 7 days at ambient temperatures. The important factor is to ensure that the media is not exposed to light at the warmer temperatures as there are vitamins and amino acids that will breakdown with light exposure.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer a methionine-free media for baculovirus expression?

Yes, we offer our methionine-free Grace's Insect Medium (Cat. No. 11595030).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (2)

引用および参考文献

Abstract

Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.

Authors:Xu L, Harada H, Yokohama-Tamaki T, Matsumoto S, Tanaka J, Taniguchi A,

Journal:J Biol Chem

PubMed ID:16293627

'Amelogenin is an extracellular matrix protein secreted by ameloblasts and is a major component of enamel matrix. Recently, in addition to their role in enamel formation, the biological activity of enamel proteins in the process of cell differentiation has recently become widely appreciated. In this study, we examined the biological ... More

A serine/threonine-rich motif is one of three nuclear localization signals that determine unidirectional transport of the mineralocorticoid receptor to the nucleus.

Authors:Walther RF, Atlas E, Carrigan A, Rouleau Y, Edgecombe A, Visentin L, Lamprecht C, Addicks GC, Haché RJ, Lefebvre YA,

Journal:J Biol Chem

PubMed ID:15737989

The mineralocorticoid receptor (MR) is a tightly regulated nuclear hormone receptor that selectively transmits corticosteroid signals. Steroid treatment transforms MR from a transcriptionally inert state, in which it is distributed equally between the nucleus and cytoplasm, to an active completely nuclear transcription factor. We report here that MR is an ... More