Grace's Insect Medium (2X), supplemented
Grace's Insect Medium (2X), supplemented
Grace's Insect Medium (2X), supplemented
Grace's Insect Medium (2X), supplemented
Gibco™

Grace's Insect Medium (2X), supplemented

Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti.詳細を見る
製品番号(カタログ番号)数量
11667037100 mL
製品番号(カタログ番号) 11667037
価格(JPY)
6,700
Each
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数量:
100 mL
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Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). This 2x formulation is intended for use in the agarose overlay when performing the plaque assay. We offer a variety of Gibco™ Media options for a range of insect cell culture applications.

This Grace's Insect Medium is modified as follows:

With
• Lactalbumin hydrolysate
• Yeastolate
• L-glutamine
• Sodium bicarbonate

The complete formulation is available. Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco™ Grace's Supplemented Insect Medium, 2x is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate, and is commonly diluted with sterile water, Fetal bovine Serum and agarose before use in the agarose overlay, as described in the product manual.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞株Sf21, Sf9
細胞タイプInsect Cell
製品ラインGibco
製品タイプGrace's Insect Medium
数量100 mL
出荷条件Room Temperature
形状Liquid
添加剤ありGlutamine, Lactalbumin Hydrolysate, Yeastolate
Unit SizeEach
組成および保存条件
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問(FAQ)

What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What medium do you recommend to grow Sf9 or Sf21 cells for performing plaque assays?

We would recommend using our Grace's Supplemented Insect Medium (Cat. No. 11667037) or SF-900 Medium (1.3X) (Cat. No. 10967032). Grace's Supplmented Insect Media is a 2X formulation intended for use in the agarose overlay when performing the plaque assay. The media has been modified with:
- Lactalbumin hydrolysate
- Yeastolate
- L-glutamine
- Sodium bicarbonate

Please go here for the complete formulation (http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.72.html). SF-900 Medium (1.3X) is a complete serum-free low protein insect cell culture medium for Sf9 and Sf21 cells, which can be used for 1% agarose overlays used for plaque assays.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.