293 SFM II

293 SFM II は、無血清および低タンパク質（安定性向上のためL-グルタミン無しで調整されています。使用前に培地1,000mlあたり、20mlの200 mM L-グルタミン詳細を見る

製品番号（カタログ番号）	数量
11686029	1000 mL

製品番号（カタログ番号） 11686029

価格（JPY）

22,600

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1000 mL

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293 SFM II は、無血清および低タンパク質（<10 μg/ml）のフォーミュラであり、高濃度の293細胞浮遊培養に最適化されています（1）。（付着293細胞培養には適応していません。）この製品は293 SFMを置き換えたものです（カタログ番号 10970-010）。 293 SFM IIには動物由来の成分が含まれていません。

安定性向上のためL-グルタミン無しで調整されています。使用前に培地1,000mlあたり、20mlの200 mM L-グルタミン、または20mlのGlutaMAX™-I 添加物を加えてください。

注： 293 培地マスターファイルは、FDAに提出済みです。マスターファイルを相互参照する許可は、テクニカルサービスまたはお近くの販売代理店にご連絡いただければ入手できます。

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

細胞株293F, 293 (HEK)

製品ラインGibco

製品タイプSerum Free Medium (SFM)

数量1000 mL

出荷条件Room Temperature

分類Animal Origin-free, Low Protein, Serum-free

Culture TypeSuspension Cell Culture

形状Liquid

Serum LevelSerum-free

添加剤なしNo Glutamine

Unit SizeEach

組成および保存条件

Store in refrigerator (2–8°C) and protect from light.

よくあるご質問（FAQ）

How do I adapt my cells to serum-free medium?

Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.

SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

Why is it necessary to gradually adapt the cells to serum-free medium?

Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (1)

引用および参考文献

Abstract

Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses.

Authors:Radoshitzky SR, Abraham J, Spiropoulou CF, Kuhn JH, Nguyen D, Li W, Nagel J, Schmidt PJ, Nunberg JH, Andrews NC, Farzan M, Choe H,

Journal:Nature

PubMed ID:17287727

'At least five arenaviruses cause viral haemorrhagic fevers in humans. Lassa virus, an Old World arenavirus, uses the cellular receptor alpha-dystroglycan to infect cells. Machupo, Guanarito, Junin and Sabia viruses are New World haemorrhagic fever viruses that do not use alpha-dystroglycan. Here we show a specific, high-affinity association between transferrin ... More