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Citations & References (3)
Invitrogen™
Platinum™ Quantitative PCR SuperMix-UDG
**********************Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve詳細を見る
製品番号（カタログ番号） 11730017
価格（JPY）お問い合わせください ›
48,400
Each
反応数:
反応100回分
**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.
**********************
Platinum™ Quantitative PCR SuperMix-UDG is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2). The system is optimized to provide superior performance with either LUX™ Fluorogenic Primers or probe-based detection systems.
Platinum™ Quantitative PCR SuperMix-UDG:
• Is highly sensitive and specific
• Allows room temperature reaction assembly (3)
• Is easy to use-just add template and fluorogenic primers or probes for detection
• Includes a vial of 50 mM MgCl2 to accommodate a variety of primer/template PCR systems
• Is compatible with most real-time instruments
Applications: Real-time quantitative amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, and fluorometric SNP (Single Nucleotide Polymorphism) detection.
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.
**********************
Platinum™ Quantitative PCR SuperMix-UDG is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2). The system is optimized to provide superior performance with either LUX™ Fluorogenic Primers or probe-based detection systems.
Platinum™ Quantitative PCR SuperMix-UDG:
• Is highly sensitive and specific
• Allows room temperature reaction assembly (3)
• Is easy to use-just add template and fluorogenic primers or probes for detection
• Includes a vial of 50 mM MgCl2 to accommodate a variety of primer/template PCR systems
• Is compatible with most real-time instruments
Applications: Real-time quantitative amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, and fluorometric SNP (Single Nucleotide Polymorphism) detection.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
反応数反応100回分
製品ラインPlatinum
製品タイプQuantitative PCR SuperMix-UDG
数量100 reactions
サンプルタイプDNA (Genomic), cDNA
出荷条件ドライアイス
対応可能対象100反応
検出法Primer-probe
使用対象（アプリケーション）遺伝子発現解析
GC-Rich PCR Performance高
PCR法qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
Platinum™ Quantitative PCR SuperMix-UDG is supplied in a 2X concentration [40 mM Tris-HCl (pH 8.4), 100 mM KCl, 6 mM MgCl2 , 400 μM dATP, 400 μM dCTP, 400 μM dGTP, 800 μM dUTP, 40 units/ml UDG, 60 units/ml Platinum™ Taq DNA Polymerase, stabilizers]. A vial of 50 mM MgCl2 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
よくあるご質問（FAQ）
Why is UDG important in the Platinum Quantitative PCR SuperMix-UDG mix?
引用および参考文献 (3)
引用および参考文献
Abstract
Localization of the secretory granule marker protein chromogranin B in the nucleus. Potential role in transcription control.
Journal:J Biol Chem
PubMed ID:11854265
'Chromogranins A (CGA) and B (CGB) are two major Ca(2+) storage proteins of the secretory granules of neuroendocrine cells. Nevertheless, we found in the present study that CGB was also localized in the nucleus. In immunogold electron microscopy using bovine adrenal medullary chromaffin cells, it was found that the number
A gnotobiotic transgenic mouse model for studying interactions between small intestinal enterocytes and intraepithelial lymphocytes.
Journal:J Biol Chem
PubMed ID:12138109
The mouse intestinal epithelium undergoes continuous renewal throughout life. Intraepithelial lymphocytes (IELs) represent a significant fraction of this epithelium and play an important role in intestinal mucosal barrier function. We have generated a germ-free transgenic mouse model to examine the effects of a genetically engineered proliferative abnormality in the principal
Floral responses to photoperiod are correlated with the timing of rhythmic expression relative to dawn and dusk in Arabidopsis.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12271123
Daylength, or photoperiod, is perceived as a seasonal signal for the control of flowering of many plants. The measurement of daylength is thought to be mediated through the interaction of phototransduction pathways with a circadian rhythm, so that flowering is induced (in long-day plants) or repressed (in short-day plants) when