SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
実際の製品は異なる場合があります
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
Invitrogen™

SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

The SuperScript™ III Platinum™ One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise詳細を見る
製品番号(カタログ番号)反応数
11736059500 Reactions
11736051反応100回分
製品番号(カタログ番号) 11736059
価格(JPY)
256,000
Each
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反応数:
500 Reactions
The SuperScript™ III Platinum™ One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. The SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection with fluorogenic primers and probes, while the SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR™ Green I detection

SuperScript™ III Platinum™ One-Step qRT-PCR Kits provide:

• SuperScript™ III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• LUX™ Fluorogenic Primers (Figure 1) or dual-labeled fluorogenic probes (Figure 2) for superior detection performance
• SYBR™ Green I dye for easy and convenient detection (Figure 3)
• Validated performance on multiple real-time instrument platforms, including rotor-based systems
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
反応数500 Reactions
ポリメラーゼTaq DNA Polymerase
製品ラインPlatinum, SYBR, SuperScript
製品タイプOne-Step qRT-PCR Kit
数量500 reactions
サンプルタイプRNA
出荷条件ドライアイス
対応可能対象500 Reactions
検出法SYBR
使用対象(アプリケーション)Real Time PCR (qPCR)
PCR法1-step RT-qPCR
反応速度スタンダード
Unit SizeEach
組成および保存条件
• 500 μL SuperScript™ III RT/Platinum™ Taq Mix
• 12.5 mL 2X SYBR™ Green Reaction Mix
• 2 × 1 mL Magnesium Sulfate (50 mM)
• 500 μL ROX Reference Dye (25 μM)
• 1.3 mL 20X Bovine Serum Albumin (1 mg/mL)

Store all components at -20°C (-80°C for long-term storage). ROX Reference Dye must be stored in the dark.

よくあるご質問(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.