SuperScript™ VILO™ cDNA Synthesis Kit

Citations & References (5)

Invitrogen™

SuperScript™ VILO™ cDNA Synthesis Kit

Name: SuperScript™ VILO™ cDNA Synthesis Kit
SKU: 11754050

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製品番号（カタログ番号）	反応数
11754050	50反応
11754250	250反応

2 オプション

製品番号（カタログ番号） 11754050

価格（JPY）

57,400

Each

お問い合わせください ›

反応数:

50反応

一括またはカスタム形式をリクエストする

SuperScript VILO cDNA合成キットは、2ステップのRT-qPCRアプリケーション用の第一鎖cDNAを生成するように設計されています。このキットは、VILO反応ミックスと酵素混合物を別々のチューブに入れた2チューブ形式で提供されます。この酵素混合物には、遺伝子操作されたMMLV RTである SuperScript III Reverse Transcriptase（RT）が含まれています。このRTはRNase H活性を低減し、熱安定性を向上させ、効率の高いcDNA合成を実現します。このキットは、幅広い量の入力RNAからcDNAを合成することができます。

注：2ステップRT-qPCRにおける優れた第一鎖cDNA合成性能を得るためには、SuperScript IV VILOマスターミックスまたはSuperScript IV VILOマスターミックス（ezDNase付属）をお勧めします。このマスターミックスは、すべてのcDNA合成反応成分を便利な1チューブマスターミックス形式で提供します。高性能で熱安定性の高いSuperScript IV RT とさらに最適化されたバッファーにより、信頼性の高いVILO技術をさらに進化させています。このマスターミックスは、より高い温度で、より短時間に効率的なcDNA合成を可能にし、最適な純度以下のテンプレートや希少なテンプレートであっても、優れたcDNA収量と感度を提供します。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

最終産物タイプcDNA（ファーストストランド）

フォーマットキット

反応数50反応

最適反応温度42℃

数量50反応

反応形態マスターミックス

試薬タイプ逆転写反応試薬

逆転写酵素SuperScript™ III

リボヌクレアーゼH活性低減

出荷条件ドライアイス

サイズ（最終製品）10 kb以下

原料RNA

技術Reverse Transcription

検出法リアルタイムPCR

使用対象（アプリケーション）Real Time PCR (qPCR)

GC-Rich PCR Performance高

反応速度高速

Unit SizeEach

組成および保存条件

• 5X VILO反応ミックス、ランダムプライマー、dNTP、およびMgCl₂を含有
• SuperScript III RT、RNaseOUT組換えリボヌクレアーゼ阻害剤、および特許取得済みヘルパータンパク質を含む10X SuperScript III酵素ブレンド

フリーザー（-5～-30℃）で保管してください。

よくあるご質問（FAQ）

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

What is the difference between SuperScript III RT and the RT in the SuperScript VILO kit?

The SuperScript VILO cDNA Synthesis Kit contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript

The SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript VILO kit is active at 42 degrees C due to the helper proteins.

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

In what concentration range of total RNA does the SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) work?

Lower limit of RNA concentration: 1.0 pg

Upper limit of RNA concentration: 2.5 µg

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center

What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

Each reverse transcription reaction requires 10 ng of DNase-treated total RNA (≥1.43 ng/µL).

View all SuperScript™ VILO™ cDNA Synthesis Kit, 50 reactions FAQs

引用および参考文献 (5)

引用および参考文献

Abstract

A useful approach to total analysis of RISC-associated RNA.

Authors:Hayashida Y, Nishibu T, Inoue K, Kurokawa T,

Journal:BMC Res Notes

PubMed ID:19706194

'ABSTRACT: BACKGROUND: Identifying the endogenous RNA induced silencing complex(RISC)-associated RNAs is essential for understanding the cellular regulatory networks by miRNAs. Recently, isolation of RISC-associated mRNAs using antibody was reported, but their method needs a large amount of initial materials. We tried to improve the protocol and constructed an efficient and ... More

Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation.

Authors:Day RC, Herridge RP, Ambrose BA, Macknight RC,

Journal:Plant Physiol

PubMed ID:18923020

During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds of mitosis without cytokinesis. This stage of endosperm development is important in determining final seed size and is a model for studying aspects of cellular and molecular biology, such as the ... More

T cells modulate Epstein-Barr virus latency phenotypes during infection of humanized mice.

Authors:Heuts F, Rottenberg ME, Salamon D, Rasul E, Adori M, Klein G, Klein E, Nagy N,

Journal:

PubMed ID:24390326

Human B cells, the main target of Epstein-Barr virus (EBV), can display several types of latent viral protein expression, denoted 0, I, IIa, IIb, or III. Of these, only type III expression induces proliferation of cells in vitro. These latency types are present at specific stages of infection and are ... More

Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.

Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,

Journal:PLoS One

PubMed ID:22393448

Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More

Cyclin D1 splice variants: polymorphism, risk, and isoform-specific regulation in prostate cancer.

Authors:Comstock CE, Augello MA, Benito RP, Karch J, Tran TH, Utama FE, Tindall EA, Wang Y, Burd CJ, Groh EM, Hoang HN, Giles GG, Severi G, Hayes VM, Henderson BE, Le Marchand L, Kolonel LN, Haiman CA, Baffa R, Gomella LG, Knudsen ES, Rui H, Henshall SM, Sutherland RL, Knudsen KE,

Journal:Clin Cancer Res

PubMed ID:19706803

PURPOSE: Alternative CCND1 splicing results in cyclin D1b, which has specialized, protumorigenic functions in prostate not shared by the cyclin D1a (full length) isoform. Here, the frequency, tumor relevance, and mechanisms controlling cyclin D1b were challenged. EXPERIMENTAL DESIGN: First, relative expression of both cyclin D1 isoforms was determined in prostate ... More