Ham's F-12 Nutrient Mix
Ham's F-12 Nutrient Mix
Citations & References (5)
Gibco™

Ham's F-12 Nutrient Mix

ハムのF-12栄養混合物（F-12）は、無血清で、チャイニーズハムスター卵（CHO）細胞の単一細胞の播種用に設計されています。F-12は、CHO培養の無血清成長や、軟骨細胞やラット前立腺細胞など詳細を見る
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製品番号（カタログ番号）数量
117650471000 mL
11765054500 mL
1176506210 x 500 mL
117650706 x 1000 mL
製品番号（カタログ番号） 11765047
価格（JPY）
5,300
Each
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数量:
1000 mL
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ハムのF-12栄養混合物（F-12）は、無血清で、チャイニーズハムスター卵（CHO）細胞の単一細胞の播種用に設計されています。F-12は、CHO培養の無血清成長や、軟骨細胞やラット前立腺細胞など、他の哺乳類細胞の血清添加成長に使用されています。当社は、幅広い細胞培養用途向けにさまざまな組成のF-12をご用意しています。培地選択ツールを使用して適切な製剤をお探しください。

このハムF-12は次のように改変されています。
含むもの
•L-グルタミン酸
•フェノールレッド

完全な組成が利用可能です。

F-12の使用
他の基本培地と比較して、F-12には亜鉛、プトレシン、ヒポキサンチン、およびチミジンなどの幅広い成分が含まれています。F-12にはタンパク質や増殖因子は含まれていませんしたがって、F-12には添加物が必要であり、通常は10%のウシ胎児血清（FBS）を使用します。F-12では炭酸水素ナトリウム緩衝系（1.176 g/L）を使用するため、生理学的pHを維持するために5–10%のCO2環境が必要です

cGMP製造および品質システム
サプライチェーン継続性のため、F-12はニューヨーク州グランドアイランドと英国スコットランドの他の施設で製造されています。どちらもcGMP製造要件に準拠した施設であり、ISO 13485認証を取得済みで、FDAに医療機器製造業者として登録されています。
仕様
細胞株CHO, COS-7, and rat prostate epithelial cells
細胞タイプPrimary Rat Astrocytes
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプHam's F-12 Nutrient Mixture
数量1000 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありGlutamine, Phenol Red, Sodium Pyruvate
添加剤なしNo HEPES
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Ham's F-12 Nutrient Mix, 1000 mL FAQs

引用および参考文献 (5)

引用および参考文献
Abstract
Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.
Authors: Takagi Y; Ninomiya H; Sakamoto A; Miwa S; Masaki T;
Journal:J Biol Chem
PubMed ID:7730310
'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More
Molecular cloning and functional analysis of the promoter of the human squalene synthase gene.
Authors: Guan G; Jiang G; Koch R L; Shechter I;
Journal:J Biol Chem
PubMed ID:7665618
'We have cloned and characterized the 5''-flanking region of the gene encoding human squalene synthase. We report here the promoter activity of successively 5''-truncated sections of a 1 kilobase of this region by fusing it to the coding region of a luciferase reporter gene. DNA segments of 200 base pairs ... More
Functional analysis of the human D2 dopamine receptor missense variants.
Authors: Cravchik A; Sibley D R; Gejman P V;
Journal:J Biol Chem
PubMed ID:8824240
The human dopamine D2 receptor gene (DRD2) has three polymorphic variants that predict the amino acid substitutions Val96 --> Ala, Pro310 --> Ser, and Ser311 --> Cys in the receptor protein. We have investigated the ligand binding and signal transduction properties of these human D2 receptor variants by stably expressing ... More
Requirement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor for selected GH-stimulated function.
Authors: Lobie P E; Allevato G; Nielsen J H; Norstedt G; Billestrup N;
Journal:J Biol Chem
PubMed ID:7665593
We have examined the involvement of tyrosine residues 333 and 338 of the growth hormone (GH) receptor in the cellular response to GH. Stable Chinese hamster ovary (CHO) cell clones expressing a receptor with tyrosine residues at position 333 and 338 of the receptor substituted for phenylalanine (CHO-GHR1-638 Y333F, Y338F) ... More
Mutational analysis of the insulin-like growth factor I prohormone processing site.
Authors: Duguay S J; Lai-Zhang J; Steiner D F;
Journal:J Biol Chem
PubMed ID:7615562
Insulin-like growth factor I (IGF-I) is a mitogenic peptide that is produced in most tissues and cell lines and plays an important role in embryonic development and postnatal growth. IGF-I is initially synthesized as a prohormone precursor that is converted to mature IGF-I by endoproteolytic removal of the carboxyl-terminal E-domain. ... More