Gateway™ pDEST™14 Vector
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Citations & References (3)
Invitrogen™

Gateway™ pDEST™14 Vector

お客様のあらゆる発現ニーズに対応するため、Invitrogenは大腸菌、昆虫、酵母、哺乳類細胞での発現や、天然型タンパク質、N末端またはC末端融合タンパク質の産出に対応する最先端のGateway™デスティネーションベクターを提供しています。すべてのGateway™デスティネーションベクターには、エントリークローンかUltimate™ RFクローンかにかかわらず詳細を見る
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製品番号（カタログ番号）数量
118010166 μg
製品番号（カタログ番号） 11801016
価格（JPY）
61,600
Each
お問い合わせください ›
数量:
6 μg
お客様のあらゆる発現ニーズに対応するため、Invitrogenは大腸菌、昆虫、酵母、哺乳類細胞での発現や、天然型タンパク質、N末端またはC末端融合タンパク質の産出に対応する最先端のGateway™デスティネーションベクターを提供しています。すべてのGateway™デスティネーションベクターには、エントリークローンかUltimate™ RFクローンかにかかわらず、任意のatt Lサイドフラグメントでの組換えのためのatt R部位があります。以下の表に、使用可能な幅広いデスティネーションベクターを示します。

必要な追加の原材料、個別に使用可能：Gateway™エントリークローン、Gateway™ LRクロナーゼ™酵素ミックス、および反応バッファー。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
抗生物質耐性菌アンピシリン（AmpR）
構成または誘導システム誘導型
製品タイプ発現ベクター
数量6 μg
選択剤（真核生物）なし
ベクターpDEST、Gateway T7ベクター
クローニング法Gateway™
製品ラインGateway、pDEST, pDEST
プロモーターT7
タンパク質タグタグなし
Unit SizeEach
組成および保存条件
すべてのデスティネーションベクターは凍結乾燥された超らせん型で提供されます。

よくあるご質問（FAQ）

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™14 Vector FAQs

引用および参考文献 (3)

引用および参考文献
Abstract
Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein.
Authors: Li Zaibo; Na Xi; Wang Dakun; Schoen Susan R; Messing Edward M; Wu Guan;
Journal:J Biol Chem
PubMed ID:11739384
'von Hippel-Lindau (VHL) disease is a hereditary cancer syndrome caused by germline mutations of the VHL gene. Recent studies suggest that VHL protein (pVHL) is a component of an E3 ubiquitin ligase, but the detailed biological function of pVHL remains to be determined. To further elucidate the biological functions of ... More
Molecular chaperone Hsp90 associates with resistance protein N and its signaling proteins SGT1 and Rar1 to modulate an innate immune response in plants.
Authors:Liu Y, Burch-Smith T, Schiff M, Feng S, Dinesh-Kumar SP,
Journal:J Biol Chem
PubMed ID:14583611
SGT1 and Rar1 are important signaling components of resistance (R) gene-mediated plant innate immune responses. Here we report that SGT1 and Rar1 associate with the molecular chaperone Hsp90. In addition, we show that Hsp90 associates with the resistance protein N that confers resistance to tobacco mosaic virus. This suggests that ... More
Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis.
Authors:Inuzuka M, Hayakawa M, Ingi T,
Journal:J Biol Chem
PubMed ID:16120614
Cell membranes contain various transporter proteins, some of which are responsible for transferring amino acids across membrane. In this study, we report another class of carrier proteins, termed Serinc1-5, that incorporates a polar amino acid serine into membranes and facilitates the synthesis of two serine-derived lipids, phosphatidylserine and sphingolipids. Serinc ... More