Gateway™ pDEST™17 Vector

Citations & References (25)

Invitrogen™

Gateway™ pDEST™17 Vector

お客様のあらゆる発現ニーズに対応するため、Invitrogenは大腸菌、昆虫、酵母、哺乳類細胞での発現や、天然型タンパク質、N末端またはC末端融合タンパク質の産出に対応する最先端のGateway™デスティネーションベクターを提供しています。すべてのGateway™デスティネーションベクターには、エントリークローンかUltimate™ RFクローンかにかかわらず詳細を見る

製品番号（カタログ番号）	数量
11803012	6 μg

製品番号（カタログ番号） 11803012

価格（JPY）

36,600

Online offer

Ends: 26-Dec-2025

61,000

割引額 24,400 (40%)

お問い合わせください ›

お客様のあらゆる発現ニーズに対応するため、Invitrogenは大腸菌、昆虫、酵母、哺乳類細胞での発現や、天然型タンパク質、N末端またはC末端融合タンパク質の産出に対応する最先端のGateway™デスティネーションベクターを提供しています。すべてのGateway™デスティネーションベクターには、エントリークローンかUltimate™ RFクローンかにかかわらず、任意のatt Lサイドフラグメントでの組換えのためのatt R部位があります。以下の表に、使用可能な幅広いデスティネーションベクターを示します。

必要な追加の原材料、個別に使用可能：
Gateway™エントリークローン、Gateway™ LR Clonase™酵素ミックス、および反応バッファー。

研究用にのみ使用できます。診断用には使用いただけません。

抗生物質耐性菌アンピシリン（AmpR）

構成または誘導システム誘導型

製品タイプ発現ベクター

数量6 μg

選択剤（真核生物）なし

ベクターpDEST、Gateway T7ベクター

クローニング法Gateway™

製品ラインGateway

プロモーターT7

タンパク質タグHisタグ（x 6）

Unit SizeEach

組成および保存条件

pDEST 17ベクターは、TEバッファー（pH 8.0）で150ng/µlで提供されます。-20℃で保存適切に保管した場合、6ヶ月間保証されます。

よくあるご質問（FAQ）

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Gateway™ pDEST™17 Vector FAQs

引用および参考文献 (25)

引用および参考文献

Abstract

Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.

Authors:Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,

Journal:J Biol Chem

PubMed ID:14699162

'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More

Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis.

Authors:Sulzenbacher G, Bignon C, Nishimura T, Tarling CA, Withers SG, Henrissat B, Bourne Y,

Journal:J Biol Chem

PubMed ID:14715651

'Fucosylated glycoconjugates are involved in numerous biological events, and alpha-l-fucosidases, the enzymes responsible for their processing, are therefore of crucial importance. Deficiency in alpha-l-fucosidase activity is associated with fucosidosis, a lysosomal storage disorder characterized by rapid neurodegeneration, resulting in severe mental and motor deterioration. To gain insight into alpha-l-fucosidase function ... More

The oncoprotein Tax binds the SRC-1-interacting domain of CBP/p300 to mediate transcriptional activation.

Authors:Scoggin KE, Ulloa A, Nyborg JK,

Journal:Mol Cell Biol

PubMed ID:11463834

'Oncogenesis associated with human T-cell leukemia virus (HTLV) infection is directly linked to the virally encoded transcription factor Tax. To activate HTLV-1 transcription Tax interacts with the cellular protein CREB and the pleiotropic coactivators CBP and p300. While extensively studied, the molecular mechanisms of Tax transcription function and coactivator utilization ... More

Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol.

Authors:Takata K, Shimizu T, Iwai S, Wood RD,

Journal:J Biol Chem

PubMed ID:16787914

'Human DNA polymerase N (POLN or pol nu) is the most recently discovered nuclear DNA polymerase in the human genome. It is an A-family DNA polymerase related to Escherichia coli pol I, human POLQ, and Drosophila Mus308. We report the first purification of the recombinant enzyme and examination of its ... More

Dual-tagging system for the affinity purification of mammalian protein complexes.

Authors:Giannone RJ, McDonald WH, Hurst GB, Huang Y, Wu J, Liu Y, Wang Y,

Journal:Biotechniques

PubMed ID:17907572

'Although affinity purification coupled with mass spectrometry (MS) provides a powerful tool to study protein-protein interactions, this strategy has encountered numerous difficulties when adapted to mammalian cells. Here we describe a Gateway-compatible dual-tag affinity purification system that integrates regulatable expression, tetracysteine motifs, and various combinations ofaffinity tags to facilitate the ... More

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