Baculovirus Expression System with Gateway™ Technology

Citations & References (3)

Invitrogen™

Baculovirus Expression System with Gateway™ Technology

製品番号（カタログ番号）	数量
11827011	20 reactions

製品番号（カタログ番号） 11827011

価格（JPY）

200,300

カートに追加

20 reactions

価格（JPY）

200,300

カートに追加

Gateway™テクノロジーを利用したBaculovirus Expression Systemたバキュロウイルス発現システムは、ポリヘドリンプロモーターを含む組換えpFastBac™プラスミドを作成するように設計されています。Gateway™テクノロジーを利用したBaculovirus Expression Systemには、以下のものが含まれます。

•Bac-to-Bac™テクノロジーを使用した発現（1）
• 天然（pDEST™8）、N末端ヒスチジン融合（pDEST™10）、およびN末端GST融合（pDEST™20）タンパク質生成用のポリヘドリンプロモーターを備えたデスティネーションベクター
• タンパク質精製後の融合タグ除去のためのTEVプロテアーゼ切断部位を含むpDEST™10ベクター（2）

研究用にのみ使用できます。診断用には使用いただけません。

製品タイプ発現システム

数量20 reactions

ベクターpDEST、Gatewayバキュロウイルスベクター

クローニング法Gateway™

製品ラインGateway

プロモーターポリヘドリン

タンパク質タグGSTタグ、Hisタグ（6x）、タグなし, His Tag (6x), Untagged

Unit SizeEach

組成および保存条件

Gateway™テクノロジーを利用したBaculovirus Expression Systemには、LR Clonase™酵素ミックスおよび反応バッファー、デスティネーションベクターpDEST™8、pDEST™10、pDEST™20、プロテイナーゼK溶液（2 μg/μl）、pENTR™-gusポジティブコントロール、およびLibrary Efficiency™ DH5コンピテントセルが含まれます。このキットには、MAX Efficiency™ DH10Bac™コンピテントセルは含まれていません。LRクロナーゼ™酵素ミックスおよびコンピテントセルを -80℃で保管してください。その他のすべての成分を-20°℃で保管してください。成分は、適切に保存されている場合、6ケ月間安定していることが保証されます。

よくあるご質問（FAQ）

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Baculovirus Expression System with Gateway™ Technology FAQs

引用および参考文献 (3)

引用および参考文献

Abstract

Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.

Authors:Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,

Journal:J Biol Chem

PubMed ID:14699162

'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More

Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.

Authors:Suzuki T, Okada Y, Semba S, Orba Y, Yamanouchi S, Endo S, Tanaka S, Fujita T, Kuroda S, Nagashima K, Sawa H,

Journal:J Biol Chem

PubMed ID:15843383

'The human polyomavirus JC virus (JCV) is the causative agent of a fatal demyelinating disease, progressive multifocal leukoencephalopathy, and encodes six major proteins, including agnoprotein. Agnoprotein colocalizes with microtubules in JCV-infected cells, but its function is not fully understood. We have now identified fasciculation and elongation protein zeta 1 (FEZ1) ... More

A novel abetalipoproteinemia genotype. Identification of a missense mutation in the 97-kDa subunit of the microsomal triglyceride transfer protein that prevents complex formation with protein disulfide isomerase.

Authors: Rehberg E F; Samson-Bouma M E; Kienzle B; Blinderman L; Jamil H; Wetterau J R; Aggerbeck L P; Gordon D A;

Journal:J Biol Chem

PubMed ID:8939939

The microsomal triglyceride transfer protein (MTP) is a heterodimer composed of the ubiquitous multifunctional protein, protein disulfide isomerase, and a unique 97-kDa subunit. Mutations that lead to the absence of a functional 97-kDa subunit cause abetalipoproteinemia, an autosomal recessive disease characterized by a defect in the assembly and secretion of ... More