RPMI 1640 Medium
RPMI 1640 Medium
Citations & References (44)
Gibco™

RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial詳細を見る
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製品番号（カタログ番号）数量
11875101100 mL
1187512720 x 100 mL
11875093500 mL
1187511910 x 500 mL
118750851000 mL
118751356 x 1000 mL
製品番号（カタログ番号） 11875101
価格（JPY）
2,100
Each
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数量:
100 mL
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red


The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
細胞株HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
細胞タイプLeukemic Cells
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
数量100 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
Serum LevelStandard Serum Supplementation
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありGlutamine, Phenol Red
添加剤なしNo HEPES, No Sodium Pyruvate
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all RPMI 1640 Medium, 100 mL FAQs

引用および参考文献 (44)

引用および参考文献
Abstract
Receptor-selective effects of endogenous RGS3 and RGS5 to regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells.
Authors:Wang Qin; Liu Min; Mullah Bashar; Siderovski David P; Neubig Richard R;
Journal:J Biol Chem
PubMed ID:12006602
Regulators of G protein signaling (RGS) proteins compose a highly diverse protein family best known for inhibition of G protein signaling by enhancing GTP hydrolysis by Galpha subunits. Little is known about the function of endogenous RGS proteins. In this study, we used synthetic ribozymes targeted to RGS2, RGS3, RGS5, ... More
Silencing of Transcription of the Human Luteinizing Hormone Receptor Gene by Histone Deacetylase-mSin3A Complex.
Authors: Zhang Ying; Dufau Maria L;
Journal:J Biol Chem
PubMed ID:12091390
'Modification of chromatin structure by histone acetylases and deacetylases is an important mechanism in modulation of eukaryotic gene transcription. The present study investigated regulation of the human luteinizing hormone receptor (hLHR) gene by histone deacetylases. Inhibition of histone deacetylases (HDACs) by trichostatin A (TSA) increased hLHR promoter activity by 40-fold ... More
Distinct Intracellular Signaling in Tumor Necrosis Factor-related Apoptosis-inducing Ligand- and CD95 Ligand-mediated Apoptosis.
Authors: Velthuis Jurjen H L; Rouschop Kasper M A; De Bont Hans J G M; Mulder Gerard J; Nagelkerke J Fred;
Journal:J Biol Chem
PubMed ID:11980895
'Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in tumor cells but not in healthy cells. Similar to CD95 ligand (CD95L), TRAIL signaling requires ligand-receptor interaction; the downstream signaling molecules, such as Fas-associated death domain and caspase-8, also seem similar. Using cells stably expressing TRAIL and ... More
Intercellular calcium signaling occurs between human osteoblasts and osteoclasts and requires activation of osteoclast P2X7 receptors.
Authors: Jørgensen Niklas R; Henriksen Zanne; Sørensen Ole H; Eriksen Erik F; Civitelli Roberto; Steinberg Thomas H;
Journal:J Biol Chem
PubMed ID:11756404
'Signaling between osteoblasts and osteoclasts is important in bone homeostasis. We previously showed that human osteoblasts propagate intercellular calcium signals via two mechanisms: autocrine activation of P2Y receptors, and gap junctional communication. In the current work we identified mechanically induced intercellular calcium signaling between osteoblasts and osteoclasts and among osteoclasts. ... More
Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process. Role of caspase-3.
Authors: Rehm Markus; Dussmann Heiko; Janicke Reiner U; Tavare Jeremy M; Kogel Donat; Prehn Jochen H M;
Journal:J Biol Chem
PubMed ID:11964393
'Activation of effector caspases is considered to be the final step in many apoptosis pathways. We transfected HeLa cells with a recombinant caspase substrate composed of cyan and yellow fluorescent protein and a linker peptide containing the caspase cleavage sequence DEVD, and we examined the cleavage kinetics at the single-cell ... More
View all RPMI 1640 Medium, 100 mL citations