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Citations & References (3)
Gibco™
DMEM, no glucose
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| 11966025 | 500 mL |
製品番号（カタログ番号） 11966025
価格（JPY）お問い合わせください ›
6,900
Each
数量:
500 mL
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
| With | Without |
| • L-glutamine | • Glucose |
| • Phenol Red | • Sodium Pyruvate |
| • HEPES |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞株HeLa, 293, Cos-7, and PC-12
細胞タイプPrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプDMEM (Dulbecco's Modified Eagle Medium)
数量500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
Serum LevelStandard Serum Supplementation
無菌性Sterile-filtered
添加剤ありGlutamine, Phenol Red
添加剤なしNo Glucose, No HEPES, No Sodium Pyruvate
Unit SizeEach
組成および保存条件
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
よくあるご質問（FAQ）
My cells will not grow in DMEM, what other type of culture media can be used with the Photoreactive Amino Acids?
What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?
引用および参考文献 (3)
引用および参考文献
Abstract
Coupling of glucose deprivation with impaired histone H2B monoubiquitination in tumors.
Journal:PLoS One
PubMed ID:22615809
Metabolic reprogramming is associated with tumorigenesis. However, glucose metabolism in tumors is poorly understood. Here, we report that glucose levels are significantly lower in bulk tumor specimens than those in normal tissues of the same tissue origins. We show that mono-ubiquitinated histone H2B (uH2B) is a semi-quantitative histone marker for
The role of p53 deacetylation in p21Waf1 regulation by laminar flow.
Journal:J Biol Chem
PubMed ID:12716906
Laminar flow arrests vascular endothelial cells at the G0/G1 phase with concurrent increase in p53 and p21Waf1. We investigated the molecular mechanism by which laminar flow activates p53 and p21Waf1 in endothelial cells. The application of a laminar flow (12 dyn/cm2) increased the deacetylation at Lys-320 and Lys-373 of p53
Glucose uptake mediated by glucose transporter 1 is essential for early tooth morphogenesis and size determination of murine molars.
Journal:Dev Biol
PubMed ID:22226978
Glucose is an essential source of energy for body metabolism and is transported into cells by glucose transporters (GLUTs). Well-characterized class I GLUT is subdivided into GLUTs1-4, which are selectively expressed depending on tissue glucose requirements. However, there is no available data on the role of GLUTs during tooth development.