DMEM, high glucose, pyruvate

Citations & References (4)

Gibco™

DMEM, high glucose, pyruvate

Name: DMEM, high glucose, pyruvate
SKU: 11995073

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.詳細を見る

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製品番号（カタログ番号）	数量
11995073	10 x 500 mL
11995065	500 mL
11995040	1000 mL
11995081	6 x 1000 mL
11995115	5 L
11995123	10 L

6 オプション

製品番号（カタログ番号） 11995073

価格（JPY）

24,200

Each

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数量:

10 x 500 mL

Customize this product

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red, Sodium pyruvate

Without: HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

仕様

細胞株HeLa, 293, Cos-7, and PC-12

細胞タイプPrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

濃度1 X

使用対象（アプリケーション）Mammalian Cell Culture

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco

製品タイプDMEM (Dulbecco's Modified Eagle Medium)

数量10 x 500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Ambient

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

添加剤ありHigh Glucose, Glutamine, Phenol Red, Sodium Pyruvate

添加剤なしNo HEPES

Unit SizeEach

組成および保存条件

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

My cells will not grow in DMEM, what other type of culture media can be used with the Photoreactive Amino Acids?

Some cells types can be adapted to grow in DMEM before using the DMEM-LM supplemented with the photoreactive amino acids. Currently we do not offer any other leucine- and methionine-depleted culture medium.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM, high glucose, pyruvate, 10 x 500 mL FAQs

引用および参考文献 (4)

引用および参考文献

Abstract

Astrocytes in culture produce anandamide and other acylethanolamides.

Authors: Walter Lisa; Franklin Allyn; Witting Anke; Moller Thomas; Stella Nephi;

Journal:J Biol Chem

PubMed ID:11916961

'Anandamide (arachidonylethanolamide) is an endocannabinoid that belongs to the acylethanolamide lipid family. It is produced by neurons in a calcium-dependent manner and acts through cannabinoid CB1 receptors. Other members of the acylethanolamide lipid family are also produced by neurons and act through G-protein-coupled receptors: homo-gamma-linolenylethanolamide (HEA) and docosatetraenylethanolamide (DEA) act ... More

PRiMA: the membrane anchor of acetylcholinesterase in the brain.

Authors: Perrier Anselme L; Massoulie Jean; Krejci Eric;

Journal:Neuron

PubMed ID:11804574

'As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. We have previously shown that collagen Q (ColQ) anchors AChE at the neuromuscular junction. We have now cloned the gene PRiMA (proline-rich membrane anchor) encoding the AChE anchor ... More

Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces.

Authors:Lavasani M, Lu A, Thompson SD, Robbins PD, Huard J, Niedernhofer LJ

Journal:Methods Mol Biol

PubMed ID:23400434

Our lab developed and optimized a method, known as the modified pre-plate technique, to isolate stem/progenitor cells from skeletal muscle. This method separates different populations of myogenic cells based on their propensity to adhere to a collagen I-coated surface. Based on their surface markers and stem-like properties, including self-renewal, multi-lineage ... More

Neuromuscular electrical stimulation as a method to maximize the beneficial effects of muscle stem cells transplanted into dystrophic skeletal muscle.

Authors:Distefano G, Ferrari RJ, Weiss C, Deasy BM, Boninger ML, Fitzgerald GK, Huard J, Ambrosio F

Journal:PLoS One

PubMed ID:23526927

Cellular therapy is a potential approach to improve the regenerative capacity of damaged or diseased skeletal muscle. However, its clinical use has often been limited by impaired donor cell survival, proliferation and differentiation following transplantation. Additionally, functional improvements after transplantation are all-too-often negligible. Because the host microenvironment plays an important ... More