CHO-S-SFM II, with hypoxanthine and thymidine
CHO-S-SFM II, with hypoxanthine and thymidine
Citations & References (3)
Gibco™

CHO-S-SFM II, with hypoxanthine and thymidine

CHO-S-SFM IIは、無血清、低タンパク質（100 μg⁄ml以下）の完全培地で、chinese hamster ovary詳細を見る
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製品番号（カタログ番号）数量
12052114500 mL
120520981 L
製品番号（カタログ番号） 12052114
価格（JPY）
18,200
Each
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数量:
500 mL
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CHO-S-SFM IIは、無血清、低タンパク質（100 μg⁄ml以下）の完全培地で、chinese hamster ovary（CHO）細胞の増殖と維持、またCHO細胞により発現した組換え型タンパク質の産生に最適化されています（1、2）。 調整済みですぐ使えるタイプの培地は、一括した継続的な潅流培養システムでCHOや他の細胞を浮遊培養するのに最適です。.

L-グルタミン含有。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞株CHO
濃度1 X
エンドトキシンレベルLow
フォーマットBottle(s)
抗生物質を含むNo antibiotics
製品ラインGibco
製品タイプSerum Free Medium (SFM)
数量500 mL
出荷条件Room Temperature
分類Low Protein, Serum-free
Culture TypeSuspension Cell Culture
形状Liquid
Serum LevelSerum-free
添加剤ありGlutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine
Unit SizeEach
組成および保存条件
Store in refrigerator (2–8°C) and protect from light.

よくあるご質問（FAQ）

Which serum-free medium should I select for my CHO cells?

Thermo Fisher Scientific offers multiple serum free media (SFM) and chemically defined (CD) media options for CHO cultures. For adherent cultures, CD CHO (10743-011) and CD OptiCHO medium (12681-011) are robust media; both are protein free and animal origin free (AOF). For adherent cultures, CD CHO-A (097-0182DJ) is a widely used protein free and animal origin free medium. There are other formulations available to suit other research needs; contact Technical Support for further information.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (3)

引用および参考文献
Abstract
Steroid ligands bind human sex hormone-binding globulin in specific orientations and produce distinct changes in protein conformation.
Authors: Grishkovskaya Irina; Avvakumov George V; Hammond Geoffrey L; Catalano Maria G; Muller Yves A;
Journal:J Biol Chem
PubMed ID:12065592
'The amino-terminal laminin G-like domain of human sex hormone-binding globulin (SHBG) contains a single high affinity steroid-binding site. Crystal structures of this domain in complex with several different steroid ligands have revealed that estradiol occupies the SHBG steroid-binding site in an opposite orientation when compared with 5 alpha-dihydrotestosterone or C19 ... More
Stable production of a human growth hormone antagonist from CHO cells adapted to serum-free suspension culture.
Authors: Haldankar R; Kopchick J J; Ridgway D;
Journal:Biotechnol Prog
PubMed ID:10356250
Human growth hormone (hGH) is a polypeptide with 191 amino acids and a molecular mass of 22 kDa. An hGH analogue was created with a single amino acid substitution (glycine[G] 120 to arginine[R]) in the third alpha-helix of the hGH molecule. This hGH analogue, named hGHG120R, was found to be ... More
Expression of human thyrotropin in cell lines with different glycosylation patterns combined with mutagenesis of specific glycosylation sites. Characterization of a novel role for the oligosaccharides in the in vitro and in vivo bioactivity.
Authors: Grossmann M; Szkudlinski M W; Tropea J E; Bishop L A; Thotakura N R; Schofield P R; Weintraub B D;
Journal:J Biol Chem
PubMed ID:7493973
We used a novel approach to study the role of the Asn-linked oligosaccharides for human thyrotropin (hTSH) activity. Mutagenesis of Asn (N) within individual glycosylation recognition sequences to Gln (Q) was combined with expression of wild type and mutant hTSH in cell lines with different glycosylation patterns. The in vitro ... More