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Invitrogen™
TRIzol™ Plus RNA Purification Kit
The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| 12183555 | 50 Preps |
製品番号（カタログ番号） 12183555
価格（JPY）お問い合わせください ›
78,600
Each
数量:
50 Preps
The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol Reagent (Cat. No. 15596026), followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink RNA Mini Kit (Cat. No. 12183020), to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.
Features of this kit include:
• Combined TRIzol-based lysis and PureLink spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol
Two products in one
The TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 μg column-;binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.
Simple lysis to column purification protocol
TRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
Features of this kit include:
• Combined TRIzol-based lysis and PureLink spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocol
Two products in one
The TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 μg column-;binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.
Simple lysis to column purification protocol
TRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used).
研究用にのみ使用できます。診断用には使用いただけません。
仕様
溶出量30 to 300 μL
最終産物タイプトータルRNA
使用対象（アプリケーション）マイクロアレイ解析、リアルタイム定量PCR（qPCR）、逆転写酵素PCR（RT-PCR）、cDNAライブラリ構築、核酸標識、ヌクレアーゼ保護アッセイ、ノーザンブロッティング
高スループット適合性ハイスループット非対応（手動）
精製時間20分
数量50 Preps
スケールミニ
出荷条件Room Temperature
出発物質量最大5x10^8個の細胞、最大200 mgの組織
収量1000 µg
Isolation Technologyシリカスピンカラム, 有機物抽出
サンプルタイプ細菌, 細胞, 植物サンプル, 組織, 酵母
Unit SizeEach
組成および保存条件
キットコンポーネント：
• TRIzol™試薬（100 ml）
• 溶解バッファー（125 ml）
• 洗浄バッファーI（50 ml）
• 洗浄バッファーII（15 ml）
• RNaseフリー水（15.5 ml）
• 収集チューブ装備のスピンカートリッジ（各50）;
• 収集チューブ（各50）;
• 回収チューブ（各50）
すべてのコンポーネントを室温で保管してください。50回のRNA単離に十分な量の試薬が付属しています。
• TRIzol™試薬（100 ml）
• 溶解バッファー（125 ml）
• 洗浄バッファーI（50 ml）
• 洗浄バッファーII（15 ml）
• RNaseフリー水（15.5 ml）
• 収集チューブ装備のスピンカートリッジ（各50）;
• 収集チューブ（各50）;
• 回収チューブ（各50）
すべてのコンポーネントを室温で保管してください。50回のRNA単離に十分な量の試薬が付属しています。
よくあるご質問（FAQ）
SDS 2.3ソフトウェアにログインするときに、ディホルト設定されている"user name"と"pass word"を教えてください？
Do you have any data showing differences in RNA extraction with TRIzol Reagent, the PureLink RNA Mini Kit, ChargeSwitch Total RNA Cell Kit or the TRIzol Plus RNA Purification Kit?
What is the difference between the TRIzol Plus RNA Purification Kit and TRIzol Reagent?
What chloroform do you recommend I use for RNA extraction with TRIzol Reagent? Are there any substitutes I can use?
What is the smallest sample volume I can use when extracting RNA with TRIzol Reagent?
引用および参考文献 (1)
引用および参考文献
Abstract
Collection and storage of human blood and adipose for genomic analysis of clinical samples.
Journal:Res Nurs Health
PubMed ID:21812005
In this methods article, we describe collection and storage of clinically acquired blood and adipose samples for transcript analysis in an ongoing study exploring obesity in renal transplant recipients. Total ribonucleic acid (RNA) was isolated from whole blood using the LeukoLOCK™ Total RNA Isolation System (n?=?4), and comparisons between fresh