PureLink™ Viral RNA/DNA Mini Kit

Citations & References (8)

Invitrogen™

PureLink™ Viral RNA/DNA Mini Kit

PureLink Viral RNA/DNA Mini Kitは、新鮮または凍結した細胞不含の生体液（血漿、血清、脳脊髄液）および細胞培養上清からウイルスRNA/DNAを同時に精製する詳細を見る

製品番号（カタログ番号）	数量
12280050	50プレップ

製品番号（カタログ番号） 12280050

価格（JPY）

52,700

お問い合わせください ›

50プレップ

PureLink Viral RNA/DNA Mini Kitは、新鮮または凍結した細胞不含の生体液（血漿、血清、脳脊髄液）および細胞培養上清からウイルスRNA/DNAを同時に精製する、迅速で効率的な方法を提供します。キットは、さまざまなRNAおよびDNAウイルスから高品質のウイルス核酸を45分以内に分離するように設計されており、低い溶出量で高感度なダウンストリーム分析が可能です。精製されたウイルスRNA／DNAは、タンパク質やヌクレアーゼを含まないため、ウイルスの検出やジェノタイピングが可能な下流のアプリケーションでの使用に適しています。

Viral RNA/DNA Mini Kitは、牛痘ウイルス、痘瘡ウイルス、サル痘ウイルス、ワクシニアウイルス、HERVなどのオルソポキウイルスからウイルスdsDNAを精製する場合に使用されています。

PureLink Viral RNA/DNA Mini Kitには、次の利点があります：
• スピンカラムを用いた遠心分離により、サンプルのクロスコンタミネーションがなく、高品質なウイルス核酸を迅速かつ効率的に精製できます
• ≤500 μLの細胞フリーサンプルからウイルスRNAおよびウイルスDNAを45分以内で精製するように設計されています。
• ウイルスの核酸を10～50 μLの低溶出量で溶出し、高感度なダウンストリーム分析が可能です

研究用にのみ使用できます。診断用には使用いただけません。

カラムタイプスピンカラム

溶出量10 to 50 μL

最終産物タイプViral RNA and DNA

使用対象（アプリケーション）リアルタイム定量PCR（qPCR）、逆転写酵素PCR（RT-PCR）、ノーザンブロッティング

高スループット適合性ハイスループット非対応（手動）

数量50プレップ

サンプルタイプ無細胞液、脳脊髄液、液体サンプル（血清など）、血漿、血清、ウイルスサンプル, Cerebrospinal Fluid, Plasma, Serum, Cell Culture Supernatants, Raw and Stabilized Saliva, Nasopharyngeal Viral Transfer Medium (NPVTM)

スケールMini

出荷条件室温

出発物質量≤500 μL

検査時間45 min

収量5 μg (Binding capacity)

Isolation Technologyシリカスピンカラム

Unit SizeEach

組成および保存条件

PureLink™ウイルスRNA／DNAミニキットには、50回の反応に十分な試薬が含まれています。

キットの内容：
• 32 ml ウイルス溶解バッファー（L22）
• 15 ml 5X 洗浄バッファー（W5）
• 2 × 1.6 ml プロテイナーゼK（保存バッファーに20 mg/ml）
• 310 µg 凍結乾燥キャリアRNA
• 15.5 ml 滅菌済み、RNAaseフリー水
• 50 ウイルススピンカラム、収集チューブ付き
• 2 × 50 洗浄チューブ（2.0 ml）
• 50 回収チューブ（1.5 ml）

キャリアRNAを除くすべての成分は室温で保存します。キャリアRNAは−20℃で保管してください。

よくあるご質問（FAQ）

Are additional wash tubes available for the PureLink Viral RNA/DNA kits if I do not want to use the wash columns twice?

Yes, the Cat. No. for 100 wash tubes is 12282-100.

What is the stability of the Proteinase K in the PureLink Viral RNA/DNA kits?

The Proteinase K solution is stable for 1 year when stored at room temperature. For long-term storage (>1 year) or if room temperature is >25 degrees C, store the Proteinase K solution at 4 degrees C.

What is the difference between serum and plasma?

Plasma is the liquid portion of the blood that is separated from the blood cells by centrifugation. One of the characteristics of plasma is that it clots easily, which is important for hemophiliacs needing a transfusion but is a nuisance in most other applications. By agitating the plasma, one can precipitate the clotting factors as a large clot, and the leftover fluid is called serum. In other words, serum plus clotting factors is plasma, and clotted plasma yields serum.

What is the stability of the carrier RNA in the PureLink Viral RNA/DNA kit, both lyophilized and in solution?

The tRNA is stable for over one year. There was no significant difference in qRT-PCR results with tRNA stored for 10 days at ‘20 degrees C or 42 degrees C. The lyophilized tRNA is stable at ‘20 degrees C, room temperature, 37 degrees C, and 45 degrees C for up to 1 month.

Do I need to use the carrier RNA in the PureLink Viral RNA/DNA kit?

The carrier RNA is tRNA and it may not be necessary. We have tested without the tRNA and obtained excellent results. The tRNA provides a substrate for RNases in the sample to protect the viral RNA until the virus is lysed. You may want to scale down the amount of carrier RNA or leave it out. However, you should validate it in your application to make sure you get the same results as when you include it.

引用および参考文献 (8)

引用および参考文献

Abstract

Surveillance of feral cats for influenza A virus in north central Florida.

Authors:Gordy JT,Jones CA,Rue J,Crawford PC,Levy JK,Stallknecht DE,Tripp RA,Tompkins SM

Journal:Influenza and other respiratory viruses

PubMed ID:22212818

Please cite this paper as: Gordy JT etÂ al. (2012) Surveillance of feral cats for influenza A virus in North Central Florida. Influenza and Other Respiratory Viruses 6(5), 341â347. Backgroundâ Transmission of highly pathogenic avian influenza and the recent pandemic H1N1 viruses to domestic cats and other felids creates concern because ... More

Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques.

Authors:Wonderlich ER,Swan ZD,Bissel SJ,Hartman AL,Carney JP,O'Malley KJ,Obadan AO,Santos J,Walker R,Sturgeon TJ,Frye LJ Jr,Maiello P,Scanga CA,Bowling JD,Bouwer AL,Duangkhae PA,Wiley CA,Flynn JL,Wang J,Cole KS,Perez DR,Reed DS,Barratt-Boyes SM

Journal:Journal of immunology (Baltimore, Md. : 1950)

PubMed ID:28062701

Human infections with highly pathogenic avian influenza A (H5N1) virus are frequently fatal but the mechanisms of disease remain ill-defined. H5N1 infection is associated with intense production of proinflammatory cytokines, but whether this cytokine storm is the main cause of fatality or is a consequence of extensive virus replication that ... More

Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors.

Authors:Kutner RH, Zhang XY, Reiser J,

Journal:Nat Protoc

PubMed ID:19300443

'Over the past decade, lentiviral vectors have emerged as powerful tools for transgene delivery. The use of lentiviral vectors has become commonplace and applications in the fields of neuroscience, hematology, developmental biology, stem cell biology and transgenesis are rapidly emerging. Also, lentiviral vectors are at present being explored in the ... More

No association of xenotropic murine leukemia virus-related virus with prostate cancer or chronic fatigue syndrome in Japan.

Authors:Furuta RA, Miyazawa T, Sugiyama T, Kuratsune H, Ikeda Y, Sato E, Misawa N, Nakatomi Y, Sakuma R, Yasui K, Yamaguti K, Hirayama F

Journal:Retrovirology

PubMed ID:21414229

The involvement of xenotropic murine leukemia virus-related virus (XMRV) in prostate cancer (PC) and chronic fatigue syndrome (CFS) is disputed as its reported prevalence ranges from 0% to 25% in PC cases and from 0% to more than 80% in CFS cases. To evaluate the risk of XMRV infection during ... More

The microbial detection array for detection of emerging viruses in clinical samples--a useful panmicrobial diagnostic tool.

Authors:Rosenstierne MW, McLoughlin KS, Olesen ML, Papa A, Gardner SN, Engler O, Plumet S, Mirazimi A, Weidmann M, Niedrig M, Fomsgaard A, Erlandsson L

Journal:

PubMed ID:24963710

Emerging viruses are usually endemic to tropical and sub-tropical regions of the world, but increased global travel, climate change and changes in lifestyle are believed to contribute to the spread of these viruses into new regions. Many of these viruses cause similar disease symptoms as other emerging viruses or common ... More

View all PureLink™ Viral RNA/DNA Mini Kit citations