OptiPRO™ SFM
OptiPRO™ SFM
Gibco™

OptiPRO™ SFM

OptiPRO™ SFMは、ウイルスおよび組換えタンパク質の産生に重要なMDCK、MDCF、VERO、BHK-21およびPK-15を含む、複数の腎臓由来細胞株の培養のためデザインされた培地です。この培地は動物由来成分を一切含有していない無血清培地です。詳細を見る
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製品番号（カタログ番号）数量
12309050100 mL
123090191000 mL
製品番号（カタログ番号） 12309050
価格（JPY）
4,800
Each
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数量:
100 mL
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OptiPRO™ SFMは、ウイルスおよび組換えタンパク質の産生に重要なMDCK、MDCF、VERO、BHK-21およびPK-15を含む、複数の腎臓由来細胞株の培養のためデザインされた培地です。この培地は動物由来成分を一切含有していない無血清培地です。
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
細胞株HeLa, MDCK, BHK-21, PK-15, COS-7, MDBK, VERO
濃度1 X
エンドトキシンレベルLow
フォーマットBottle(s)
抗生物質を含むNo antibiotics
製品ラインGibco
製品タイプSerum Free Medium
数量100 mL
出荷条件Room Temperature
分類Animal Origin-free, Low Protein, Serum-free
Culture TypeAdherent Cell Culture
形状Liquid
Serum LevelSerum-free
添加剤ありPhenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine
添加剤なしNo Glutamine
Unit SizeEach
組成および保存条件
Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

Do you offer a protocol for titering virus in OptiPRO SFM?

For virus titering in OptiPRO SFM we recommend using Falcon or equivalent 96-well plates and plating cells at a concentration of 1 X 10^6/mL. If you are having trouble growing cells for virus titration in OptiPRO SFM in the 96-well plates, we suggest switching to the EMEM with 5%FBS or DMEM with 5% FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a recommended protocol for culturing cell lines in OptiPRO SFM that require sequential adaptation?

The sequential adaptation process is a multi-subculture of cells. We recommend the following passaging sequence:
• First passage: use 25% OptiPRO SFM:75% original stock medium
• Second passage: use 50% OptiPRO SFM:50% original stock medium
• Third passage: use 75% OptiPRO SFM:25% original stock medium
• Fourth and subsequent passages: use 100% OptiPRO SFM

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I add antibiotics to OptiPRO SFM?

You can supplement OptiPRO SFM with 5mL/L Antibiotic-Antimycotic (Cat. No. 15240096)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What concentration of L-glutamine or GlutaMAX-I should I add to OptiPRO SFM before I use it?

We recommend adding L-glutamine or GlutaMAX-I to OptiPRO SFM at a final concentration of 4mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.