McCoy's 5A (Modified) Medium, HEPES

Citations & References (2)

Gibco™

McCoy's 5A (Modified) Medium, HEPES

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell詳細を見る

製品番号（カタログ番号）	数量
12330031	500 mL

製品番号（カタログ番号） 12330031

価格（JPY）

4,200

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500 mL

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McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

With	Without
• High Glucose	• Sodium Pyruvate
• L-glutamine
• Bacto-peptone
• Phenol Red
• HEPES

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO₂ incubator.

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For human ex vivo tissue and cell culture processing applications.

細胞株Rat fibroblasts

細胞タイプBiopsy Tissues

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco

製品タイプMcCoy's 5A Modified Medium

数量500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin

形状Liquid

無菌性Sterile-filtered

添加剤ありHigh Glucose, Glutamine, HEPES, Phenol Red

添加剤なしNo Sodium Pyruvate

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all McCoy's 5A (Modified) Medium, HEPES FAQs

引用および参考文献 (2)

引用および参考文献

Abstract

A potential H-DNA element in the MUC1 promoter does not influence transcription.

Authors: Pahwa G S; Maher L J 3rd; Hollingsworth M A;

Journal:J Biol Chem

PubMed ID:8900124

A purine/pyrimidine mirror repeat element (M-PMR3) in the MUC1 promoter has been shown to form H-DNA under in vitro conditions. We investigated this element for biological function in the regulation of transcription of this gene. Chloramphenicol acetyltransferase reporter-promoter constructs were prepared in which the mirror repeat element (PMR3) was intact, ... More

B-Raf is dispensable for K-Ras-mediated oncogenesis in human cancer cells.

Authors:Kim JS, Lee C, Foxworth A, Waldman T,

Journal:Cancer Res

PubMed ID:15026326

Oncogenic mutations in B-Raf and Kirsten-Ras (K-Ras) are mutually exclusive during human cancer pathogenesis. In an effort to study the biological basis of this epistasis, gene targeting was used to create isogenic sets of human cancer cells differing only in presence or absence of endogenous oncogenic K-Ras or wild-type B-Raf. ... More