293fectin™ Transfection Reagent
293fectin™ Transfection Reagent
Citations & References (3)
Gibco™

293fectin™ Transfection Reagent

293fectin™トランスフェクション試薬は、DNAを真核細胞にトランスフェクションするための特許取得済みの陽イオン脂質ベースの組成です。この試薬は、定義済みの懸濁液293ヒト胚性腎細胞（フリースタイル293 F細胞、カタログ番号 R790-07）、無血清フリースタイル™293発現培地詳細を見る
テクニカルサポートオーダーサポート
表示を変更buttonViewtableView
製品番号（カタログ番号）数量
123470191 mL
12347500
または、製品番号12347-500
15 mL
12347750
または、製品番号12347-750
10 x 15 mL
製品番号（カタログ番号） 12347019
価格（JPY）
84,600
Each
お問い合わせください ›
数量:
1 mL
293fectin™トランスフェクション試薬は、DNAを真核細胞にトランスフェクションするための特許取得済みの陽イオン脂質ベースの組成です。この試薬は、定義済みの懸濁液293ヒト胚性腎細胞（フリースタイル293 F細胞、カタログ番号 R790-07）、無血清フリースタイル™293発現培地（カタログ番号12338～018）をトランスフェクションし、フリースタイル293発現システム（カタログ番号K9000-01）。293fectin™トランスフェクション試薬には次の利点があります。

•懸濁液293細胞で高いトランスフェクション効率を発揮し、接着293細胞のトランスフェクションにも適しています
• 懸濁フリースタイル™293～F細胞は、フリースタイル™293発現培地でトランスフェクションできます。培地の交換は不要です
• 293fectin™試薬複合体を培養培地の細胞に直接加えます
•トランスフェクション後に複合体を除去したり、培地を変更または追加する必要はありません。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象（アプリケーション）Transfection
高スループット適合性ハイスループット非対応（手動）
製品ライン293fectin
製品タイプトランスフェクション試薬
数量1 mL
適合血清可
細胞タイプ株化細胞株
サンプルタイププラスミドDNA
Transfection Technique脂質ベースのトランスフェクション
Unit SizeEach
組成および保存条件
各ユニットには試薬のバイアルが1本含まれています。4℃で保存。293fectin™は、適切に保存した場合、6カ月間安定した状態で保たれます。

よくあるご質問（FAQ）

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all 293fectin™ Transfection Reagent, 1 mL FAQs

引用および参考文献 (3)

引用および参考文献
Abstract
Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.
Authors:Ido H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM, Sekiguchi K,
Journal:J Biol Chem
PubMed ID:14701821
'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought ... More
Binding of anthrax toxin to its receptor is similar to alpha integrin-ligand interactions.
Authors:Bradley KA, Mogridge J, Jonah G, Rainey A, Batty S, Young JA,
Journal:J Biol Chem
PubMed ID:14507921
The secreted protein toxin produced by Bacillus anthracis contributes to virulence of this pathogen and can cause many of the symptoms seen during an anthrax infection, including shock and sudden death. The cell-binding component of anthrax toxin, protective antigen, mediates entry of the toxin into cells by first binding directly ... More
T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface.
Authors:Cao E, Zang X, Ramagopal UA, Mukhopadhaya A, Fedorov A, Fedorov E, Zencheck WD, Lary JW, Cole JL, Deng H, Xiao H, Dilorenzo TP, Allison JP, Nathenson SG, Almo SC,
Journal:Immunity
PubMed ID:17363302
The T cell immunoglobulin mucin (Tim) family of receptors regulates effector CD4(+) T cell functions and is implicated in autoimmune and allergic diseases. Tim-3 induces immunological tolerance, and engagement of the Tim-3 immunoglobulin variable (IgV) domain by galectin-9 is important for appropriate termination of T helper 1-immune responses. The 2 ... More