DMEM, high glucose, HEPES

Citations & References (3)

Gibco™

DMEM, high glucose, HEPES

Name: DMEM, high glucose, HEPES
SKU: 12430054

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.詳細を見る

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製品番号（カタログ番号）	数量
12430054	500 mL
12430062	10 x 500 mL
12430047	1000 mL
12430104	6 x 1000 mL
12430112 または、製品番号12430-112	5 L

5 オプション

製品番号（カタログ番号） 12430054

価格（JPY）

4,700

Each

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数量:

500 mL

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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• Sodium Pyruvate
• L-glutamine
• HEPES
• Phenol Red

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

仕様

細胞株HeLa, 293, Cos-7, and PC-12

細胞タイプPrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

濃度1 X

使用対象（アプリケーション）Mammalian cell culture

グルコース濃度4500 mg/L

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco

製品タイプDMEM (Dulbecco's Modified Eagle Medium)

数量500 mL

品質保持期間12 Months From Date of Manufacture

出荷条件Room Temperature

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

添加剤ありHigh Glucose, Glutamine, HEPES, Phenol Red

添加剤なしNo Sodium Pyruvate

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM, high glucose, HEPES, 500 mL FAQs

引用および参考文献 (3)

引用および参考文献

Abstract

The connecting segment between both epidermal growth factor-like domains in blood coagulation factor IX contributes to stimulation by factor VIIIa and its isolated A2 domain.

Authors:Celie Patrick H N; Van Stempvoort Gunny; Fribourg Caroline; Schurgers Leon J; Lenting Peter J; Mertens Koen;

Journal:J Biol Chem

PubMed ID:11925427

The light chain of activated factor IX comprises multiple interactions between both epidermal growth factor-like domains that contribute to enzymatic activity and binding of factor IXa to its cofactor factor VIIIa. To investigate the association between factor IXa-specific properties and surface-exposed structure elements, chimeras were constructed in which the interconnection ... More

Thiazolidinedione activation of peroxisome proliferator-activated receptor gamma can enhance mitochondrial potential and promote cell survival.

Authors: Wang Y Lynn; Frauwirth Kenneth A; Rangwala Shamina M; Lazar Mitchell A; Thompson Craig B;

Journal:J Biol Chem

PubMed ID:12082115

'Thiazolidinediones (TZDs) are widely used for treatment of type 2 diabetes mellitus. Peroxisome proliferator-activated receptor gamma (PPAR gamma) is the molecular target of TZDs and is believed to mediate the apoptotic effects of this class of drugs in a variety of cell types, including B and T lymphocytes. The finding ... More

Activation of the proteolytic activity of ADAMTS4 (aggrecanase-1) by C-terminal truncation.

Authors: Gao Gui; Westling Jennifer; Thompson Vivian P; Howell Troy D; Gottschall Paul E; Sandy John D;

Journal:J Biol Chem

PubMed ID:11796708

'Proteolysis of the hyalectans (aggrecan, versican, brevican) in vivo appears to result from the activity of ADAMTS4 (aggrecanase-1, herein referred to as an hyalectanase). To examine the mode of activation of ADAMTS4, a human chondrosarcoma cell line, JJ012, has been stably transfected with the full-length c-DNA for human ADAMTS4. The ... More