IMDM
IMDM
Citations & References (1)
Gibco™

IMDM

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.詳細を見る
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製品番号（カタログ番号）数量
1244006110 x 500 mL
12440053500 mL
124400461000 mL
124400796 x 1000 mL
製品番号（カタログ番号） 12440061
価格（JPY）
32,200
Each
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数量:
10 x 500 mL
Customize this product

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a variety of IMDM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This IMDM is modified as follows:

With: L-glutamine, Phenol Red

Without: α-thioglycerol, 2-mercaptoethanol

The complete formulation is available.

Using IMDM

IMDM, a modification of Dulbecco's Modified Eagle Medium, includes selenium as well as additional amino acids and vitamins. In addition, this unique medium lacks iron, with potassium nitrate replacing ferric nitrate. IMDM contains no proteins, lipids, or growth factors. Therefore, IMDM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system (3.024 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
細胞株Jurkat, COS-7, and macrophage cells
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプIMDM (Iscove's Modified Dulbecco's Medium)
数量10 x 500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin-free
形状Liquid
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありHigh Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
添加剤なしNo α-Thioglycerol, No 2-Mercaptoethanol
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

Is IMDM (Cat. No. 12440061) also available without folic acid or folate?

Unfortunately, we do not offer any supplements without folic acid or folate. We would recommend going to this page (https://www.thermofisher.com/us/en/home/global/forms/biopro-info-gibco-bioprocessing.html) and filling out the form to request a quote for a custom IMDM, if feasible, made to your specifications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all IMDM, 10 x 500 mL FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Ex vivo differentiation of cord blood stem cells into megakaryocytes and platelets.
Authors:Pineault N, Robert A, Cortin V, Boyer L
Journal:Methods Mol Biol
PubMed ID:23179834
Megakaryocytes (MK) are hematopoietic cells present in the bone marrow that are responsible for the production and release of platelets in the circulation. Given their very low frequency (<1%), human MK often need to be derived in culture to study their development or to generate sufficient material for biological studies. ... More