Advanced DMEM
Advanced DMEM
Citations & References (1)
Gibco™

Advanced DMEM

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
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製品番号（カタログ番号）数量
12491015500 mL
1249102310 x 500 mL
製品番号（カタログ番号） 12491015
価格（JPY）
4,900
Each
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数量:
500 mL
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Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

This Advanced DMEM is manufactured as follows:

With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine

The complete formulation is available.

Using Advanced DMEM

Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞株MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
濃度1 X
製造品質cGMP-compliant under the ISO 13485 standard
製品ラインGibco
製品タイプDMEM (Dulbecco's Modified Eagle Medium)
数量500 mL
品質保持期間12 Months From Date of Manufacture
出荷条件Room Temperature
分類Animal Origin
形状Liquid
Serum LevelReduced Serum
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
添加剤ありHigh Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
添加剤なしNo Glutamine, No HEPES
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

What kind of ascorbic acid is present in Advanced DMEM (Cat. No. 12491015 or 12491023)?

Advanced DMEM contains 2.5 mg/L of ascorbic acid phosphate to maintain the stability. You do not need to supplement it with anything else.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Advanced DMEM Medium and other DMEM formulations?

Advanced Media are widely used basal media, which require 50-90% less Fetal Bovine Serum (FBS) supplementation compared to conventional basal media formulations. The Advanced media is unique due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all Advanced DMEM FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.
Authors:Vacanti V, Kong E, Suzuki G, Sato K, Canty JM, Lee T,
Journal:J Cell Physiol
PubMed ID:15880640
'The in vitro culture of porcine bone marrow-derived mesenchymal stem cells (MSCs) was used for the investigation of adult stem cell biology. Isolated porcine MSCs possessed the ability to proliferate extensively in Gibco(R) Advanced DMEM containing 5% fetal bovine serum (FBS). Greater than 40 serial MSC passages and 100 cell ... More