AmnioMAX™ C-100 Complete Medium

Citations & References (5)

Gibco™

AmnioMAX™ C-100 Complete Medium

AmnioMAX™-C100は、初代ヒト羊水細胞の培養用に適した製品です。注意：旧カタログ# 12558-029と同じ内容です。AmnioMAX™-C100 完全培地は、 AmnioMAX™-C100 基礎培地詳細を見る

製品番号（カタログ番号）	数量
12558011	1 Set

製品番号（カタログ番号） 12558011

価格（JPY）

14,900

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1 Set

AmnioMAX™-C100は、初代ヒト羊水細胞の培養用に適した製品です。
注意：旧カタログ# 12558-029と同じ内容です。

AmnioMAX™-C100 完全培地は、 AmnioMAX™-C100 基礎培地（カタログ# 17001-140）とAmnioMAX™-C100 添加物（カタログ#12556-031）から構成されています。添加材には抗生物質と増殖促進物質が添加されており、さらなる添加物は必要はありません。培地と添加剤は単品でもお求めいただけます。

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AmnioMAX™-C100 基礎培地は、アミノサイトの高い増殖率と付着が可能となります。絨毛膜サンプルおよび羊水サンプルで良好な結果が得られます。完全培地として使用、あるいはMEM Alphaとウシ胎児血清で希釈して使用できます。

AmnioMAX™-C100 Supplement添加物には、適量の抗生物質と増殖促進物質が含まれており、さらなる添加物の追加は必要ありません。

AmnioMAX™-C100 培地の品質管理検査については、初代羊水分離物を用いた6日間のコロニーアッセイが行われています。 AmnioMAX™ 基礎培地とAmnioMAX™ 添加物の各ロットには、平均コロニー数、平均分析可能コロニー数、100細胞ごとの分裂指数などを記した分析証明書（COA）が付属しています。

For in vitro diagnostic use.

細胞タイプAmniocyte Cells

抗生物質を含むGentamicin

製品ラインAmnioMAX

製品タイプComplete Medium

数量1 Set

品質保持期間16 Months

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

添加剤ありGlutamine

Unit SizeEach

組成および保存条件

Basal Medium (90 ml):
Storage: 2 – 8°C
Ships: Ambient temperature
Shelf life: 16 months from date of manufacture

Supplement (15 ml):
Storage: -5 to -20°C
Ships: Frozen
Shelf life: 16 months from date of manufacture

Use complete medium within 7–10 days once opened.

引用および参考文献 (5)

引用および参考文献

Abstract

Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.

Authors:Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19482945

'The innovation of reprogramming somatic cells to induced pluripotent stem cells provides a possible new approach to treat beta-thalassemia and other genetic diseases such as sickle cell anemia. Induced pluripotent stem (iPS) cells can be made from these patients'' somatic cells and the mutation in the beta-globin gene corrected by ... More

Reactivation of XIST in normal fibroblasts and a somatic cell hybrid: abnormal localization of XIST RNA in hybrid cells.

Authors:Hansen RS, Canfield TK, Stanek AM, Keitges EA, Gartler SM,

Journal:Proc Natl Acad Sci U S A

PubMed ID:9560241

'The XIST gene, expressed only from the inactive X chromosome, is a critical component of X inactivation. Although apparently unnecessary for maintenance of inactivation, XIST expression is thought to be sufficient for inactivation of genes in cis even when XIST is located abnormally on another chromosome. This repression appears to ... More

Specific tumour-associated methylation in normal human term placenta and first-trimester cytotrophoblasts.

Authors:Novakovic B, Rakyan V, Ng HK, Manuelpillai U, Dewi C, Wong NC, Morley R, Down T, Beck S, Craig JM, Saffery R,

Journal:Mol Hum Reprod

PubMed ID:18708652

'Human placentation displays many similarities with tumourigenesis, including rapid cell division, migration and invasion, overlapping gene expression profiles and escape from immune detection. Recent data have identified promoter methylation in the Ras association factor and adenomatous polyposis coli tumour suppressor genes as part of this process. However, the extent of ... More

Placenta-specific methylation of the vitamin D 24-hydroxylase gene: implications for feedback autoregulation of active vitamin D levels at the fetomaternal interface.

Authors:Novakovic B, Sibson M, Ng HK, Manuelpillai U, Rakyan V, Down T, Beck S, Fournier T, Evain-Brion D, Dimitriadis E, Craig JM, Morley R, Saffery R,

Journal:J Biol Chem

PubMed ID:19237542

'Plasma concentrations of biologically active vitamin D (1,25-(OH)(2)D) are tightly controlled via feedback regulation of renal 1alpha-hydroxylase (CYP27B1; positive) and 24-hydroxylase (CYP24A1; catabolic) enzymes. In pregnancy, this regulation is uncoupled, and 1,25-(OH)(2)D levels are significantly elevated, suggesting a role in pregnancy progression. Epigenetic regulation of CYP27B1 and CYP24A1 has previously ... More

Development of transgenic cloned pig models of skin inflammation by DNA transposon-directed ectopic expression of human ß1 and a2 integrin.

Authors:Staunstrup NH, Madsen J, Primo MN, Li J, Liu Y, Kragh PM, Li R, Schmidt M, Purup S, Dagnæs-Hansen F, Svensson L, Petersen TK, Callesen H, Bolund L, Mikkelsen JG,

Journal:PLoS One

PubMed ID:22590584

Integrins constitute a superfamily of transmembrane signaling receptors that play pivotal roles in cutaneous homeostasis by modulating cell growth and differentiation as well as inflammatory responses in the skin. Subrabasal expression of integrins a2 and/or ß1 entails hyperproliferation and aberrant differentiation of keratinocytes and leads to dermal and epidermal influx ... More