MEM α, nucleosides

Citations & References (4)

Gibco™

MEM α, nucleosides

Name: MEM α, nucleosides
SKU: 12571071

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative詳細を見る

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製品番号（カタログ番号）	数量
12571071	10 x 500 mL
12571063	500 mL
12571048	1000 mL
12571089	6 x 1000 mL

4 オプション

製品番号（カタログ番号） 12571071

価格（JPY）

42,000

Each

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数量:

10 x 500 mL

Customize this product

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With

• Ribonucleosides

• Deoxyribonucleosides

• Phenol Red

• L-glutamine

The complete formulation is available.

Using MEM α
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

仕様

細胞株HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

細胞タイプPrimary Rat Astrocytes

濃度1 X

製造品質cGMP-compliant under the ISO 13485 standard

製品ラインGibco

製品タイプMEM α (Minimum Essential Medium α)

数量10 x 500 mL

品質保持期間12 Months From Date of Manufacture

分類Animal Origin-free

形状Liquid

Serum LevelStandard Serum Supplementation

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

添加剤ありLow Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides

添加剤なしNo HEPES

Unit SizeEach

組成および保存条件

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

What is the difference between MEM α, nucleosides and MEM α, no nucleosides?

Our MEM α, nucleosides is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Our MEM α without nucleosides can be used a selection medium for DG44 and other DHFR-negative cells (DHFR = dihydrofolate reductase).
DHFR-negative cells are unable to grow unless the culture medium is supplemented with essential metabolites, including hypoxanthine and thymidine.

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM α, nucleosides, 10 x 500 mL FAQs

引用および参考文献 (4)

引用および参考文献

Abstract

Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells.

Authors:Cheng Z, Ito S, Nishio N, Xiao H, Zhang R, Suzuki H, Okawa Y, Murohara T, Isobe K

Journal:J Mol Cell Biol

PubMed ID:21228011

'If induced pluripotent stem (iPS) cells are to be used to treat damaged tissues or repair organs in elderly patients, it will be necessary to establish iPS cells from their tissues. To determine the feasibility of using this technology with elderly patients, we asked if it was indeed possible to ... More

A high-throughput automated platform for the development of manufacturing cell lines for protein therapeutics.

Authors:Shi S, Condon RG, Deng L, Saunders J, Hung F, Tsao YS, Liu Z

Journal:J Vis Exp

PubMed ID:21968840

'The fast-growing biopharmaceutical industry demands speedy development of highly efficient and reliable production systems to meet the increasing requirement for drug supplies. The generation of production cell lines has traditionally involved manual operations that are labor-intensive, low-throughput and vulnerable to human errors. We report here an integrated high-throughput and automated ... More

Specific inhibition of ICAM-1 expression mediated by gene targeting with Triplex-forming oligonucleotides.

Authors:Besch R, Giovannangeli C, Kammerbauer C, Degitz K

Journal:J Biol Chem

PubMed ID:12080053

Selected sequences in the DNA double helix can be specifically recognized by oligonucleotides via hydrogen bonding interactions. The resulting triple helix can modulate DNA metabolism and especially interfere with transcription in a gene-specific manner. To explore the potential of triplex-forming oligonucleotides (TFOs) as gene repressors, a TFO was designed to ... More

Expression of human thyrotropin in cell lines with different glycosylation patterns combined with mutagenesis of specific glycosylation sites. Characterization of a novel role for the oligosaccharides in the in vitro and in vivo bioactivity.

Authors: Grossmann M; Szkudlinski M W; Tropea J E; Bishop L A; Thotakura N R; Schofield P R; Weintraub B D;

Journal:J Biol Chem

PubMed ID:7493973

We used a novel approach to study the role of the Asn-linked oligosaccharides for human thyrotropin (hTSH) activity. Mutagenesis of Asn (N) within individual glycosylation recognition sequences to Gln (Q) was combined with expression of wild type and mutant hTSH in cell lines with different glycosylation patterns. The in vitro ... More