TrypLE™ Express Enzyme (1X), phenol red
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TrypLE™ Express Enzyme (1X), phenol red
TrypLE™ Express Enzyme (1X), phenol red
TrypLE™ Express Enzyme (1X), phenol red
Citations & References (1)
Gibco™

TrypLE™ Express Enzyme (1X), phenol red

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接着哺乳類細胞をプラスチックから解離させるための動物トリプシンに代わる組換え酵素。TrypLE™ Expressは、 TrypLE™ Selectで見られる酵素と同じものになりますが、生産規模をより経済的なものにして製造されています。TrypLE™ Expressは冷蔵で保存することができます。また室温でも6ヶ月間安定です詳細を見る
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製品番号（カタログ番号）数量
12605010100 mL
1260503620 x 100mL
126050935 L
12605028500 mL
製品番号（カタログ番号） 12605010
価格（JPY）
5,100
Each
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数量:
100 mL
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接着哺乳類細胞をプラスチックから解離させるための動物トリプシンに代わる組換え酵素。TrypLE™ Expressは、 TrypLE™ Selectで見られる酵素と同じものになりますが、生産規模をより経済的なものにして製造されています。TrypLE™ Expressは冷蔵で保存することができます。また室温でも6ヶ月間安定です。 TrypLE™ Expressは幅広い細胞に作用し、トリプシンのプロトコルを変更する必要はありません。TrypLE™ ExpressはPBS-EDTA バッファーの1X濃度で提供され、フェノールレッドを含有しています。

研究目的またはさらに発展した細胞培養を利用した製造目的での使用。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞タイプMammalian Cells
キレート剤EDTA
濃度1 X
培養タイプAdherent Cell Culture
グリーン機能Energy efficient, Sustainable packaging
浸透圧Check and Record
製品ラインGibco
数量100 mL
備考Room Temperature Stable
品質保持期間24 Months
出荷条件Room Temperature
電子線源Animal origin-free
試験済みIn vitro Bioassay
分類Animal Origin-free
形状Liquid
製品タイプCell Culture Dissociation Reagent
無菌性Sterile-filtered
添加剤ありPhenol Red, EDTA
Unit SizeEach
組成および保存条件
Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What enzymes do you recommend for passaging mesenchymal stem cells (MSC)?

We recommend using TrypLE Express Enzyme or TrypLE Select Enzyme for passaging mesenchymal stem cells (MSCs). The usage of trypsin will result in lower cell viability upon passage. TrypLE Express and TrypLE Select are defined, animal origin-free, and stable at both room and refrigerated temperatures.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does TrypLE Express Enzyme have a His-tag?

TrypLE Express Enzyme does not have a His-tag

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?

There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For your TrypLE products, how does the enzyme get inactivated?

Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:

After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all TrypLE™ Express Enzyme (1X), phenol red, 100 mL FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
A simple tool to improve pluripotent stem cell differentiation.
Authors:Chetty S, Pagliuca FW, Honore C, Kweudjeu A, Rezania A, Melton DA,
Journal:Nat Methods
PubMed ID:23584186
We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced ... More