TrypLE™ Express Enzyme (1X), phenol red

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Citations & References (3)

Gibco™

TrypLE™ Express Enzyme (1X), phenol red

Name: TrypLE™ Express Enzyme (1X), phenol red
SKU: 12605028

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製品番号（カタログ番号）	数量
12605028	500 mL
12605010	100 mL
12605036	20 x 100mL
12605093	5 L

4 オプション

製品番号（カタログ番号） 12605028

価格（JPY）

13,700

Each

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数量:

500 mL

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接着哺乳類細胞をプラスチックから解離させるための動物トリプシンに代わる組換え酵素。TrypLE™ Expressは、 TrypLE™ Selectで見られる酵素と同じものになりますが、生産規模をより経済的なものにして製造されています。TrypLE™ Expressは冷蔵で保存することができます。また室温でも6ヶ月間安定です。 TrypLE™ Expressは幅広い細胞に作用し、トリプシンのプロトコルを変更する必要はありません。TrypLE™ ExpressはPBS-EDTA バッファーの1X濃度で提供され、フェノールレッドを含有しています。

研究目的またはさらに発展した細胞培養を利用した製造目的での使用。

研究用途にのみご使用ください。診断目的には使用できません。

仕様

細胞タイプMammalian Cells

キレート剤EDTA

濃度1 X

培養タイプAdherent Cell Culture

グリーン機能Energy efficient, Sustainable packaging

浸透圧Check and Record

製品ラインGibco

数量500 mL

備考Room Temperature Stable

品質保持期間24 Months

出荷条件Room Temperature

電子線源Animal origin-free

試験済みIn vitro Bioassay

分類Animal Origin-free

形状Liquid

製品タイプCell Culture Dissociation Reagent

無菌性Sterile-filtered

添加剤ありPhenol Red, EDTA

Unit SizeEach

組成および保存条件

Storage conditions: 15°C to 30°C, protect from light
Alternate storage conditions: 2°C to 8°C or -5°C to -20°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does TrypLE Express Enzyme have a His-tag?

TrypLE Express Enzyme does not have a His-tag

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?

There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For your TrypLE products, how does the enzyme get inactivated?

Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:

After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, with and without phenol red? What does the phenol red do?

The difference between the two products is just the presence or absence of phenol red. Phenol red is a pH indicator. You can use either product to dissociate your cells. When you use TrypLE Express enzyme to dissociate cells, they very likely become single cells. If you want to passage cells as clumps, you may consider using other reagents such as EDTA.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all TrypLE™ Express Enzyme (1X), phenol red, 500 mL FAQs

引用および参考文献 (3)

引用および参考文献

Abstract

Directed differentiation of pancreatic δ cells from human pluripotent stem cells.

Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H

Journal:Nature communications

PubMed ID:39068220

Dysfunction of pancreatic Î´ cells contributes to the etiology of diabetes. Despite their important role, human Î´ cells are scarce, limiting physiological studies and drug discovery targeting Î´ cells. To date, no directed Î´-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More

Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.

Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV

Journal:Frontiers in immunology

PubMed ID:39007148

BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More

A simple tool to improve pluripotent stem cell differentiation.

Authors:Chetty S, Pagliuca FW, Honore C, Kweudjeu A, Rezania A, Melton DA,

Journal:Nat Methods

PubMed ID:23584186

We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced ... More