Sf-900™ III SFM

Citations & References (2)

Gibco™

Sf-900™ III SFM

Name: Sf-900™ III SFM
SKU: 12658019

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製品番号（カタログ番号）	数量
12658019	500 mL
12658027	1000 mL
12658035	10 L
12658001	20 L

4 オプション

製品番号（カタログ番号） 12658019

価格（JPY）

8,300

Each

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数量:

500 mL

Customize this product

Sf-900™ III SFMは、加水分解物が少なく、無タンパク質で、動物由来成分を含まない培地で、細胞増殖における一貫性と信頼性とを提供します。この培地は動物由来成分を含まないため、迷入因子をスクリーニングする必要が少なくなります。

バキュロウイルス発現系および安定昆虫発現系のどちらの系を使用した場合でも、懸濁培養におけるSf9およびSf21細胞の増殖および組換え遺伝子発現をサポートおよび最大化するよう最適化されています。
Sf9細胞の増殖を10-14 x 106細胞/mlの密度までサポートします。
適切に保存した場合、12ヶ月間安定であることを保証します。

保存:
遮光した場所に2から8°Cで保存します。適切に保存した場合、安定性は12ヶ月間保証されます。

研究用途にのみご使用ください。診断目的には使用できません。

仕様

細胞株Sf21, Sf9

細胞タイプInsect Cell

製品ラインGibco, Sf-900

製品タイプInsect Cell Serum Free Medium (SFM)

数量500 mL

出荷条件Room Temperature

種S. frugiperda, Spodoptera frugiperda

分類Animal Origin-free, Protein-free, Serum-free

形状Liquid

Serum LevelSerum-free

添加剤ありGlutamine

Unit SizeEach

組成および保存条件

Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

よくあるご質問（FAQ）

What antimicrobials can be used in insect culture and at what concentration?

Many antibiotics are suitable for use with insect cells. The following antibiotics are commonly used:
- Penicillin/Streptomycine: 50-100 U/mL; 50-100 µg/mL
- Amphotericin B (Fungizone antimycotic): 0.25 µg/mL
- Gentamicin: 0.5 mL of 10 mg/mL solution in 500 mL media (final concentration: 10 µg/mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to heat-inactivate my serum before adding it to my medium?

Heat inactivation is not necessary. Our team has routinely used serum that has not been heat-inactivated, and we have not observed any effect on cell growth or morphology.

Many cells do not require heat-inactivated FBS. Some cells prefer heat-inactivated FBS. For instance, we use heat-inactivated FBS for our insect cell lines, i.e., Sf9 and Sf21 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer serum-free insect media?

Yes, we offer a variety of serum-free insect media. Please go here (http://www.thermofisher.com/us/en/home/life-science/cell-culture/insect-cell-culture/insect-cell-culture-misc/serum-free-media.html?icid=cvc-insect-media-c2t2) to view the media we offer and the differences between them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (2)

引用および参考文献

Abstract

Predictors of seropositivity to human papillomavirus type 53: one of the most prevalent high risk-related cervical human papillomaviruses.

Authors:Malik ZA, Hailpern SM, Burk RD,

Journal:Viral Immunol

PubMed ID:18681800

'Persistent cervicovaginal infection with high-risk types of HPV is the major risk factor for subsequent cervical neoplasia. HPV53, part of the alpha 6 species group along with HPV types 30, 56, and 66, is one of the most prevalent high risk-related HPV types, yet little is known about the molecular ... More

The S100A8-serum amyloid A3-TLR4 paracrine cascade establishes a pre-metastatic phase.

Authors:Hiratsuka S, Watanabe A, Sakurai Y, Akashi-Takamura S, Ishibashi S, Miyake K, Shibuya M, Akira S, Aburatani H, Maru Y,

Journal:Nat Cell Biol

PubMed ID:18820689

A large number of macrophages and haematopoietic progenitor cells accumulate in pre-metastatic lungs in which chemoattractants, such as S100A8 and S100A9, are produced by distant primary tumours serving as metastatic soil. The exact mechanism by which these chemoattractants elicit cell accumulation is not known. Here, we show that serum amyloid ... More