Bovine Pituitary Extract (BPE)

ウシ下垂体抽出物の各ロットのタンパク質濃度は、Bradford法により決定されます。BPEの各バイアルに含まれるタンパク質量は25 mg未満です。詳細を見る

製品番号（カタログ番号）	数量
13028014	25 mg

製品番号（カタログ番号） 13028014

価格（JPY）

16,900

お問い合わせください ›

25 mg

ウシ下垂体抽出物の各ロットのタンパク質濃度は、Bradford法により決定されます。BPEの各バイアルに含まれるタンパク質量は25 mg未満です。

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプMammalian Cells

培養タイプMammalian Cell Culture

数量25 mg

品質保持期間18 Months

出荷条件Dry Ice

形状Liquid

製品タイプBovine Pituitary Extract

pH6 to 8

Unit SizeEach

組成および保存条件

Store in freezer (-5 to -30°C).

よくあるご質問（FAQ）

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (1)

引用および参考文献

Abstract

Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling.

Authors:Utani A, Momota Y, Endo H, Kasuya Y, Beck K, Suzuki N, Nomizu M, Shinkai H,

Journal:J Biol Chem

PubMed ID:12826666

'The LG4 module of the laminin alpha 3 chain (alpha 3 LG4), a component of epithelial-specific laminin-5, has cell attachment activity and binds syndecan (Utani, A., Nomizu, M., Matsuura, H., Kato, K., Kobayashi, T., Takeda, U., Aota, S., Nielsen, P. K., and Shinkai, H. (2001) J. Biol. Chem. 276, 28779-28788). ... More