Cell Dissociation Buffer, enzyme-free, PBS
Cell Dissociation Buffer, enzyme-free, PBS
Citations & References (3)
Gibco™

Cell Dissociation Buffer, enzyme-free, PBS

Gibco™細胞解離バッファーは、カルシウムフリーおよびマグネシウムフリーのリン酸緩衝化生理食塩水（PBS）に入っている、薄膜でろ過された、等浸透圧の酵素フリーの塩溶液、キレート剤、細胞調製剤です。Gibco™細胞解離バッファーは、リガンド結合詳細を見る
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製品番号（カタログ番号）数量
13151014100 mL
製品番号（カタログ番号） 13151014
価格（JPY）
7,600
Each
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数量:
100 mL
Gibco™細胞解離バッファーは、カルシウムフリーおよびマグネシウムフリーのリン酸緩衝化生理食塩水（PBS）に入っている、薄膜でろ過された、等浸透圧の酵素フリーの塩溶液、キレート剤、細胞調製剤です。Gibco™細胞解離バッファーは、リガンド結合、フローサイトメトリー、免疫組織化学など、無傷の細胞表面タンパク質を必要とする研究における哺乳類細胞の穏やかな解離に適しています。Gibco™細胞解離バッファーは、HeLaやNIH 3T3などの軽接着細胞株に適していますが、強接着細胞のルーチン継代には推奨されません。解離プロトコルは、当社のテクニカルリファレンスライブラリでご利用いただけます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞株HeLa, NIH 3T3
キレート剤EDTA
数量100 mL
備考Room Temperature Stable
品質保持期間24 Months
出荷条件Room Temperature
分類Animal Origin-free
製品タイプCell Culture Dissociation Reagent
無菌性Sterile-filtered
添加剤ありEDTA
Unit SizeEach
組成および保存条件
Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

What is the osmolality and pH range for Cell Dissociation Buffer, enzyme-free, PBS (Cat. No. 13151014)

Below are QC test specifications for pH and osmolality for this product:

- pH: 6.9 to 7.9
- Osmolality: 289 to 321 mOsm/kg

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

At what confluency should cells be at prior to their dissociation using the Cell Association Buffer, enzyme-free, PBS?

The buffer should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, it will be hard to detach them.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will the Cell Dissociation Buffer, enzyme-free, PBS damage cell surface markers/receptors?

This buffer is very gentle. It should preserve the structural and functional integrity of cell surface proteins. However, we recommend that the customer test their cells and proteins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use the Cell Dissociation Buffer, enzyme-free, PBS to dissociate cells?

This Enzyme-Free Cell Dissociation Buffer does not rely on proteolysis for its action. Therefore, it is not suitable for strongly adherent cells, or for routine passage of some cell types (e.g., A431, Hep2, A549, MDCK, WI-38). This product is intended to gently dissociate cells from attachment substrates and each other while maintaining surface protein integrity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

引用および参考文献 (3)

引用および参考文献
Abstract
Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.
Authors:Choi KD, Vodyanik M, Slukvin II,
Journal:Nat Protoc
PubMed ID:21372811
'In this paper, we describe a protocol for hematopoietic differentiation of human pluripotent stem cells (hPSCs) and generation of mature myeloid cells from hPSCs through expansion and differentiation of hPSC-derived lin(-)CD34(+)CD43(+)CD45(+) multipotent progenitors. The protocol comprises three major steps: (i) induction of hematopoietic differentiation by coculture of hPSCs with OP9 ... More
Long term non-invasive imaging of embryonic stem cells using reporter genes.
Authors:Sun N, Lee A, Wu JC,
Journal:Nat Protoc
PubMed ID:19617890
'Development of non-invasive and accurate methods to track cell fate after delivery will greatly expedite transition of embryonic stem (ES) cell therapy to the clinic. In this protocol, we describe the in vivo monitoring of stem cell survival, proliferation and migration using reporter genes. We established stable ES cell lines ... More
Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice.
Authors: Marron Michele P; Graser Robert T; Chapman Harold D; Serreze David V;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12361980
Particular major histocompatibility complex (MHC) class II alleles clearly contribute to T cell-mediated autoimmune type 1 diabetes (T1D) in both humans and nonobese diabetic (NOD) mice. However, studies in NOD mice indicate MHC class I-restricted T cell responses are also essential to T1D development. In humans, epidemiological studies have suggested ... More