HBSS, calcium, magnesium, no phenol red
HBSS, calcium, magnesium, no phenol red
Gibco™

HBSS, calcium, magnesium, no phenol red

ハンクス平衡塩類溶液(HBSS)は、解離前の細胞の洗浄、細胞や組織サンプルの輸送、計数時の細胞の希釈、試薬の調製など、さまざまな細胞培養アプリケーションに使用します。カルシウムとマグネシウムを含む製剤は、一般的に輸送培地として使用するか詳細を見る
製品番号(カタログ番号)数量
14025092500 mL
1402513410 x 500 mL
140250761,000 mL
140251266 x 1000 mL
製品番号(カタログ番号) 14025092
価格(JPY)
3,300
Each
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数量:
500 mL
Customize this product
ハンクス平衡塩類溶液(HBSS)は、解離前の細胞の洗浄、細胞や組織サンプルの輸送、計数時の細胞の希釈、試薬の調製など、さまざまな細胞培養アプリケーションに使用します。カルシウムとマグネシウムを含む製剤は、一般的に輸送培地として使用するか、試薬の調製に使用します。

当社は、幅広い細胞培養アプリケーションに対応するさまざまな Gibco™ HBSS製剤 を提供しています。

このHBSSは次のように変更しています:

含むもの含まないもの
•カルシウム•フェノールレッド
•マグネシウム•ピルビン酸ナトリウム
• グルコース

詳細な 組成 を利用できます。

cGMP製造品質システム
Gibco™ HBSSは、ニューヨーク州グランドアイランドのcGMP適合施設で製造しています。この施設は医療機器製造業者としてFDAに登録されており、ISO 13485の認証を受けています。サプライチェーン継続性のために、当社のスコットランド施設(14065-049)で製造された同等のGibco™ HBSS製品を提供しています。この施設は医療機器製造業者としてFDAに登録されており、ISO 13485の認証を受けています。
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
化学物質名または材質ハンクス平衡塩類溶液(HBSS)
消去
内容No Sodium Pyruvate
製造品質cGMP-compliant under the ISO 13485 standard
浸透圧270∼305 mOsm/kg
推奨保存方法保存条件:15–30°C
輸送条件:室温
保存期間:製造日から36カ月
無菌性滅菌ろ過済み
濃度1 X
使用対象(アプリケーション)哺乳類細胞培養
物理的フォームLiquid
製品ラインGibco
数量500 mL
溶液タイプハンクス平衡塩類
pH6.7
Unit SizeEach

よくあるご質問(FAQ)

What is the pH of HBSS, calcium, magnesium, no phenol red at room temperature.

At room temperature, HBSS, calcium, magnesium, no phenol red has a pH of 6.7 - 7.8.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I need to add additional HEPES to Hank's Balanced Salt Solution (HBSS) (Cat. No. 14025xxx)?

HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.