An alternative processing of integrin alpha(v) subunit in tumor cells by membrane type-1 matrix metalloproteinase.
Authors: Ratnikov Boris I; Rozanov Dmitri V; Postnova Tanya I; Baciu Peter G; Zhang Heying; DiScipio Richard G; Chestukhina Galina G; Smith Jeffrey W; Deryugina Elena I; Strongin Alex Y;
Journal:J Biol Chem
PubMed ID:11741954
'Membrane type-1 matrix metalloproteinase (MT1-MMP) and alpha(v)beta(3) integrin are both essential to cell invasion. Maturation of integrin pro-alpha(v)chain (pro-alpha(v)) involves its cleavage by proprotein convertases (PC) to form the disulfide-bonded 125-kDa heavy and 25-kDa light alpha chains. Our report presents evidence of an alternative pathway of pro-alpha(v) processing involving MT1-MMP. ... More
An evaluation of a four-channel multiplexed electrospray tandem mass spectrometry for higher throughput quantitative analysis.
Authors: Morrison Denise; Davies Amy E; Watt Alan P;
Journal:Anal Chem
PubMed ID:11985324
A four-channel multiplexed electrospray inlet system (MUX) coupled to a triple quadrupole mass spectrometer was investigated as a higher throughput approach to quantitative analysis. Four discrete samples may be simultaneously analyzed by virtue of a rotating sampler with a concomitant 4-fold increase in analytical throughput. Although absolute sensitivity was reduced ... More