Nunc™ Cell Culture Inserts in Carrier Plate Systems

The membrane is approximately 1.0 mm above the bottom of the dish for the Nunc Polycarbonate Cell Culture Inserts.

Due to the large pore size of the Nunc polycarbonate inserts, spots or holes are observed and they represent the membrane. In this plane of focus, the most visible cells are the ones located on the membrane, whereas the slightly blurred backdrop are the cells on the dish that are in a different plane of focus. These cells do not photograph with great resolution due to the slightly opaque quality of the membrane. However, these cells are visible in culture.

Low position: 2 mL (12-well), 1 mL (24-well), Medium position: 3 mL (12-well), 1.5 mL (24-well), High position: 4 mL (12-well), 2 mL (24-well). Some optimization may be necessary for development purposes.

Standard culture membrane inserts are placed directly into the wells of a Multidish, and the feet at the bottom of the insert maintain a distance of about 1 mm between the membrane and the Multidish culture surface. The “carrier plate” culture membrane product adds a tray between the Multidish and the lid, with a set of hanger brackets at each well. These brackets engage one of three sets of tabs on each insert, to allow for selection between 3 different distances (approximately 1, 3, or 6 mm) between the membrane and the Multidish culture surface. This can allow for more flexibility in experiments, such as distance-dependent interaction between populations or media volume under cultures at the air-media interface.