HBSS, no calcium, no magnesium, no phenol red
HBSS, no calcium, no magnesium, no phenol red
Citations & References (1)
Gibco™

HBSS, no calcium, no magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,詳細を見る
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製品番号（カタログ番号）数量
14175095500 mL
1417510310 x 500 mL
141750791000 mL
141751456 x 1000 mL
製品番号（カタログ番号） 14175095
価格（JPY）
3,300
Each
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数量:
500 mL
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.


This HBSS is modified as follows:
WithWithout
• Glucose• Calcium
 • Magnesium
 • Phenol Red


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
化学物質名または材質ハンクス平衡塩類溶液(HBSS)
色消去
内容No Sodium Pyruvate
製造品質cGMP-compliant under the ISO 13485 standard
浸透圧270 - 295 mOsm/kg
推奨保存方法保存条件：15∼8°C
出荷条件：室温
有効期限：製造日から36カ月
無菌性滅菌ろ過済み
濃度1 X
使用対象（アプリケーション）哺乳類細胞培養
物理的フォームLiquid
製品ラインGibco
数量500 mL
溶液タイプハンクス平衡塩類
pH6.7 - 7.8
Unit SizeEach

よくあるご質問（FAQ）

Do you offer a 10X version of the HBSS, no calcium, no magnesium, no phenol red (Cat. No. 14175095)?

Yes, we do offer HBSS (10X), no calcium, no magnesium, no phenol red (Cat. No. 14185052).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (1)

引用および参考文献
Abstract
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Authors:Avci FY, Li X, Tsuji M, Kasper DL,
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More