DPBS, no calcium, no magnesium
DPBS, no calcium, no magnesium
Citations & References (1)
Gibco™

DPBS, no calcium, no magnesium

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing詳細を見る
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製品番号（カタログ番号）数量パッケージ
141902356 x 1000 mLBottle
14190144500 mLBottle
1419025010 x 500 mLBottle
141901361000 mLBottle
141903425 LBag
1419035910 LBag
1419036720 LBag
製品番号（カタログ番号） 14190235
価格（JPY）
24,400
Each
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数量:
6 x 1000 mL
パッケージ:
Bottle
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Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco™ DPBS formulations for a range of cell culture applications.

This DPBS is modified as follows:

Without: Calcium, Magnesium, Phenol Red

The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
仕様
化学物質名または材質DPBS
色消去
内容No Sodium Pyruvate
製造品質cGMP-compliant under the ISO 13485 standard
浸透圧270∼300 mOsm/kg
パッケージBottle
推奨保存方法保存条件：15∼8°C
出荷条件：室温
有効期限：製造日から36カ月
無菌性滅菌ろ過済み
濃度1 X
使用対象（アプリケーション）哺乳類細胞培養
物理的フォームLiquid
製品ラインGibco
数量6 x 1000 mL
溶液タイプダルベッコリン酸緩衝生理食塩水
pH7.0 to 7.3
Unit SizeEach

よくあるご質問（FAQ）

What is the shelf life of DPBS after it has been opened?

We cannot provide an official expiration date for open bottles as this depends on the customer's use. Once opened, Thermo Fisher Scientific can no longer guarantee the performance of any product. However, if the storage conditions are followed as recommended, the stated expiration date (36 months from the date of manufacture) should apply to opened bottles as DPBS is extremely stable.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of DPBS (Cat. No. 14190136)?

DPBS has a shelf life of 36 months from the date of manufacture, when stored as recommended (15-30 degrees C).

Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?

Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DPBS, no calcium, no magnesium, 6 x 1000 mL bottle FAQs

引用および参考文献 (1)

引用および参考文献
Abstract
Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns.
Authors:Sanchez-Freire V, Ebert AD, Kalisky T, Quake SR, Wu JC,
Journal:Nat Protoc
PubMed ID:22481529
'Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles at a molecular level in approximately 11 h after cell sample collection. We present here a high-content microfluidic real-time platform as a powerful tool for comparatively investigating the regulation of developmental processes in single ... More