Penicillin-Streptomycin (5,000 U/mL)

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Citations & References (23)

Gibco™

Penicillin-Streptomycin (5,000 U/mL)

抗生物質のペニシリンとストレプトマイシンは、グラム陽性細菌とグラム陰性細菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。ペニシリンは当初、真菌青カビから精製され、細菌細胞壁の回転を直接的に妨害することで作用し、また細胞壁をさらに変える酵素の放出を誘発することにより間接的に作用します。ストレプトマイシンは、ストレプトミセスグリセウスから精製されました。細菌リボソームの30Sサブユニットに結合することで作用し詳細を見る

製品番号（カタログ番号）	数量
15070063	100 mL

製品番号（カタログ番号） 15070063

価格（JPY）

6,900

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100 mL

抗生物質のペニシリンとストレプトマイシンは、グラム陽性細菌とグラム陰性細菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。ペニシリンは当初、真菌青カビから精製され、細菌細胞壁の回転を直接的に妨害することで作用し、また細胞壁をさらに変える酵素の放出を誘発することにより間接的に作用します。ストレプトマイシンは、ストレプトミセスグリセウスから精製されました。細菌リボソームの30Sサブユニットに結合することで作用し、タンパク質の合成および感受性細菌の死亡を阻害します。この溶液には、5000ユニット／mlのペニシリンと5000 µgのストレプトマイシンが含まれています。

当社は、粉末および液体の両方のフォーマットで、幅広い抗生物質および抗真菌剤を提供しています。詳細なリストはこちらをご覧ください。または以下の製品を検索してください。

•汚染防止
• 真核生物および細菌の選択

選択性抗生物質の推奨使用濃度をご覧ください。

細胞培養における抗生物質および抗真菌剤の使用の詳細と、培養の汚染除去に関するガイドラインをご覧ください。

研究用にのみ使用できます。診断用には使用いただけません。

濃度100 X

培養タイプMammalian Cell Culture, Insect Cell Culture

使用対象（アプリケーション）Prevention of Cell Culture Contamination

数量100 mL

品質保持期間12 Months

出荷条件Dry Ice

形状Liquid

製品タイプAntibiotic

無菌性Sterile-filtered

Unit SizeEach

組成および保存条件

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (23)

引用および参考文献

Abstract

Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.

Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; BÃƒÂ¶hm Michael; Nickenig Georg;

Journal:Circulation

PubMed ID:12021231

BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More

Directed differentiation of pancreatic δ cells from human pluripotent stem cells.

Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H

Journal:Nature communications

PubMed ID:39068220

Dysfunction of pancreatic Î´ cells contributes to the etiology of diabetes. Despite their important role, human Î´ cells are scarce, limiting physiological studies and drug discovery targeting Î´ cells. To date, no directed Î´-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More

Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.

Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,

Journal:J Biol Chem

PubMed ID:22810232

'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More

A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production.

Authors: Watters Jyoti J; Sommer Julie A; Pfeiffer Zachary A; Prabhu Usha; Guerra Alma N; Bertics Paul J;

Journal:J Biol Chem

PubMed ID:11786532

'Endotoxin (lipopolysaccharide, LPS) is a component of the outer membrane of Gram-negative bacteria and promotes the activation of macrophages and microglia. Although these cells are highly LPS-responsive, they serve unique tissue-specific functions and exhibit different LPS sensitivities. Accordingly, it was of interest to evaluate whether these biological differences reside in ... More

Electrophoretic profiling of both RNA and protein from a single 250-pL sample.

Authors: Zabzdyr Jennifer L; Lillard Sheri J;

Journal:Anal Chem

PubMed ID:11985318

'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based ... More

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