Trypsin (2.5%), no phenol red

Citations & References (3)

Gibco™

Trypsin (2.5%), no phenol red

Gibco Trypsin is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive詳細を見る

製品番号（カタログ番号）	数量
15090046	100 mL

製品番号（カタログ番号） 15090046

価格（JPY）

11,100

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100 mL

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Gibco Trypsin is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE reagent that feature:

• Quality Testing
• Documented Traceability
• Dual-site cGMP Manufacturing

The complete formulation is available.

Quality Testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, the raw materials are verified for e-beam irradiation and precursor lots are tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco Trypsin (2.5%), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.

研究または製造用途です。診断目的には使用できません。

細胞タイプMammalian Cells

キレート剤No EDTA

濃度10 X

浸透圧270 - 320 mOsm/kg

製品ラインGibco

数量100 mL

品質保持期間24 Months

出荷条件Wet Ice

電子線源Animal Origin

試験済みIn vitro Bioassay

分類Animal Origin

形状Liquid

製品タイプCell Culture Dissociation Reagent

無菌性Sterile-filtered

添加剤なしNo Phenol Red

pH7.1 to 8

Unit SizeEach

組成および保存条件

Storage conditions: -5°C to -20°C
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

How long can I keep trypsin in the refrigerator after thawing?

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you have a non-animal-origin enzyme for cell dissociation?

Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

View all Trypsin (2.5%), no phenol red FAQs

引用および参考文献 (3)

引用および参考文献

Abstract

Inhibition of transforming growth factor beta signaling and Smad-dependent activation of transcription by the Latent Membrane Protein 1 of Epstein-Barr virus.

Authors: Prokova Vassiliki; Mosialos George; Kardassis Dimitris;

Journal:J Biol Chem

PubMed ID:11781310

'Inhibition of transforming growth factor beta (TGFbeta) signaling by the Epstein-Barr virus Latent Membrane Protein 1 (LMP1) may account, at least in part, for the oncogenic activity of LMP1. We found that LMP1 is a potent inhibitor of TGFbeta signaling and Smad-dependent activation of transcription in 293 epithelial cells and ... More

TRPC4 Can Be Activated by G-protein-coupled Receptors and Provides Sufficient Ca2+ to Trigger Exocytosis in Neuroendocrine Cells.

Authors: Obukhov Alexander G; Nowycky Martha C;

Journal:J Biol Chem

PubMed ID:11856742

Transient receptor potential (TRP) channels form a large family of plasma membrane cation channels. Mammalian members of the

A conditional knockout resource for the genome-wide study of mouse gene function.

Authors:Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A,

Journal:Nature

PubMed ID:21677750

Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in ... More