Antibiotic-Antimycotic (100X)
Antibiotic-Antimycotic (100X)
Citations & References (13)
Gibco™

Antibiotic-Antimycotic (100X)

Gibco™抗生物質-抗真菌剤は、細菌汚染および真菌汚染を防止するために使用されます。この溶液には、10,000ユニット／mLのペニシリン、10,000 µg/mLのストレプトマイシン、および25 µg/mLのGibcoアンフォテリシンBが含まれています。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により詳細を見る
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製品番号（カタログ番号）数量
15240062100 mL
1524009620 mL
1524011220 x 100 mL
製品番号（カタログ番号） 15240062
価格（JPY）
11,900
Each
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数量:
100 mL
Gibco™抗生物質-抗真菌剤は、細菌汚染および真菌汚染を防止するために使用されます。この溶液には、10,000ユニット／mLのペニシリン、10,000 µg/mLのストレプトマイシン、および25 µg/mLのGibcoアンフォテリシンBが含まれています。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。アムホテリシンBは、多細胞性菌類および酵母の阻害により、細胞培養の真菌汚染を防止します。

ペニシリンは菌類青カビから独自に精製され、細菌細胞壁の代謝回転を直接的に妨害することで作用し、細胞壁をさらに変化させる酵素の放出を誘発することによって間接的に作用します。ストレプトマイシンは、ストレプトマイセスグリセウスから精製されました。細菌リボソームの30Sサブユニットに結合することで作用し、タンパク質合成を阻害し、感受性細菌を死滅させます。アムホテリシンBは、真菌性血漿膜透過性を高めることで真菌類や酵母の成長を防止する抗真菌剤です。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
濃度100 X
培養タイプMammalian Cell Culture
数量100 mL
品質保持期間12 Months
出荷条件Dry Ice
形状Liquid
製品タイプAntibiotic-Antimycotic
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
Unit SizeEach
組成および保存条件
Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

Can I use pen/strep or other antibiotics for episomal reprogramming of cells?

The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the solvent used in Antibiotic-Antimycotic (100X)?

The components are in a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the components of Antibiotic-Antimycotic (100X)?

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition for Antibiotic-Antimycotic (100X)?

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Antibiotic-Antimycotic (100X) FAQs

引用および参考文献 (13)

引用および参考文献
Abstract
Common variation at 6q16 within HACE1 and LIN28B influences susceptibility to neuroblastoma.
Authors:Diskin SJ, Capasso M, Schnepp RW, Cole KA, Attiyeh EF, Hou C, Diamond M, Carpenter EL, Winter C, Lee H, Jagannathan J, Latorre V, Iolascon A, Hakonarson H, Devoto M, Maris JM,
Journal:Nat Genet
PubMed ID:22941191
Neuroblastoma is a cancer of the sympathetic nervous system that accounts for approximately 10% of all pediatric oncology deaths. Here, we report a genome-wide association study of 2,817 neuroblastoma cases and 7,473 controls. We identified two new associations at 6q16, the first within HACE1 (rs4336470; combined P=2.7×10(-11); odds ratio 1.26, ... More
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Single-Cell Transcriptome Atlas of Murine Endothelial Cells.
Authors:Kalucka J, de Rooij LPMH, Goveia J, Rohlenova K, Dumas SJ, Meta E, Conchinha NV, Taverna F, Teuwen LA, Veys K, García-Caballero M, Khan S, Geldhof V, Sokol L, Chen R, Treps L, Borri M, de Zeeuw P, Dubois C, Karakach TK, Falkenberg KD, Parys M, Yin X, Vinckier S, Du Y, Fenton RA, Schoonjans L, Dewerchin M, Eelen G, Thienpont B, Lin L, Bolund L, Li X, Luo Y, Carmeliet P
Journal:Cell
PubMed ID:32059779
'The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7' ... More
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