Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?
Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.
Bombyxin is a growth factor for wing imaginal disks in Lepidoptera.
Authors: Nijhout H Frederik; Grunert Laura W;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12429853
'The mechanisms that control the growth rate of internal tissues during postembryonic development are poorly understood. In insects, the growth rate of imaginal disks varies with nutrition and keeps pace with variation in somatic growth. We describe here a mechanism by which the growth of wing imaginal disks is controlled. ... More
Apoptosis induces efflux of the mitochondrial matrix enzyme deoxyguanosine kinase.
Authors:Jüllig M, Eriksson S,
Journal:J Biol Chem
PubMed ID:11294860
Deoxyguanosine kinase (dGK) initiates the salvage of purine deoxynucleosides in mitochondria and is a key enzyme in mitochondrial DNA precursor synthesis. The active form of the enzyme is a 60-kDa protein normally located in the mitochondrial matrix. Here we describe the subcellular distribution of dGK during apoptosis in human epithelial ... More
Hypoxia and Nitric Oxide Treatment Confer Tolerance to Glucose Starvation in a 5'-AMP-activated Protein Kinase-dependent Manner.
Hypoxia is a critical event for higher organisms, and cells and tissues react by increasing the oxygen supply by vasodilatation, angiogenesis, and erythropoiesis and maintaining cellular energy by increasing glycolysis and inhibiting anabolic pathways. Stimulation of glycolysis has been regarded as the main response that increases energy production during hypoxia; ... More
Metabolic stress activates an ERK/hnRNPK/DDX3X pathway in pancreatic ß cells.
Authors:Good AL, Haemmerle MW, Oguh AU, Doliba NM, Stoffers DA
Journal:Mol Metab
PubMed ID:31178390
Pancreatic ß cell failure plays a central role in the development of type 2 diabetes (T2D). While the transcription factors shaping the ß cell gene expression program have received much attention, the post-transcriptional controls that are activated in ß cells during stress are largely unknown. We recently identified JUND as ... More
PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations.
Authors:Cartwright IM, Haskins JS, Kato TA
Journal:Methods Mol Biol
PubMed ID:31267424
Dicentric and centric ring chromosomes are used for radiation-induced damage analysis and biodosimetry after radiation exposure. However, Giemsa stain-based cytogenetic analysis is labor-intense and time-consuming. Moreover, the disadvantage of Giemsa based chromosome analysis is a potential poor reproducibility when researchers are not fully trained for analysis. These problems come from ... More