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Citations & References (3)
Gibco™
Trypan Blue Solution, 0.4%
Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test.詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| 15250061 | 100 mL |
製品番号（カタログ番号） 15250061
価格（JPY）お問い合わせください ›
4,300
Each
数量:
100 mL
Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately. The dye exclusion test is based upon the concept that viable cells do not take up impermeable dyes (like Trypan Blue), but dead cells are permeable and take up the dye.
cGMP manufacturing and quality system
Trypan Blue Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
cGMP manufacturing and quality system
Trypan Blue Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
細胞タイプMammalian Cells
色Blue
濃度0.004
検出法Colorimetric
使用対象（アプリケーション）Cell Counting
数量100 mL
品質保持期間36 Months
出荷条件Room Temperature
形状Liquid
製品タイプTrypan Blue
無菌性Sterile-filtered
Unit SizeEach
組成および保存条件
Storage conditions: 15 – 30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
よくあるご質問（FAQ）
How do I quench any fluorescence outside of my live cells and leave my intracellular staining intact?
How is Gibco Trypan Blue Solution, 0.4% (Cat. No. 15250061) sterilized?
What is Trypan Blue Solution, 0.4% dissolved in?
How long can cells be exposed to trypan blue before all cells are stained with the dye?
What causes trypan blue to form a precipitate?
引用および参考文献 (3)
引用および参考文献
Abstract
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic âlivers in a dish.â We describe strategies to induce
Bax and Bak independently promote cytochrome C release from mitochondria.
Journal:J Biol Chem
PubMed ID:11836241
'Pro-apoptotic Bax and Bak have been implicated in the regulation of p53-dependent apoptosis. We assessed the ability of primary baby mouse kidney (BMK) epithelial cells from bax(-/-), bak(-/-), and bax(-/-) bak(-/-) mice to be transformed by E1A alone or in conjunction with dominant-negative p53 (p53DD). Although E1A alone transformed BMK
Hypoxia and Nitric Oxide Treatment Confer Tolerance to Glucose Starvation in a 5'-AMP-activated Protein Kinase-dependent Manner.
Journal:J Biol Chem
PubMed ID:12091379
Hypoxia is a critical event for higher organisms, and cells and tissues react by increasing the oxygen supply by vasodilatation, angiogenesis, and erythropoiesis and maintaining cellular energy by increasing glycolysis and inhibiting anabolic pathways. Stimulation of glycolysis has been regarded as the main response that increases energy production during hypoxia;