X-Gal

Name: X-Gal
SKU: 15520018

β-ガラクトシターゼの基質であるX-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside)は、加水分解時に青色の沈殿物を産出するため、免疫ブロッティングおよび免疫細胞化学的分析の基質として使用されます。 β-ガラクトシダーゼ遺伝子を持つ細菌が、X-galを含むプレート上で増殖すると、青色のコロニーを産出します。 X-galは白色の結晶上でお届けします詳細を見る

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製品番号（カタログ番号）	数量	製品タイプ
15520018	1 g	X-Gal Substrate
15520034	100 mg	X-Gal Substrate

2 オプション

製品番号（カタログ番号） 15520018

価格（JPY）

96,600

Each

お問い合わせください ›

数量:

1 g

製品タイプ:

X-Gal Substrate

研究用途にのみご使用ください。診断目的には使用できません。

仕様

化学物質名または材質5-bromo-4-chloro-3-indolyl-β-D-galactoside

概要X-Gal, Cell-Permeant

使用対象（アプリケーション）Immunoblotting

製品タイプX-Gal Substrate

数量1 g

出荷条件Approved for shipment at Room Temperature or on Wet Ice

検出法Colorimetric

形状Powder

フォーマットBottle

Unit SizeEach

組成および保存条件

• X-Gal, powder, 1 g

よくあるご質問（FAQ）

Can X-gal be resuspended in dimethylsulfoxide (DMSO) instead of dimethylformamide (DMF)? How are X-gal plates prepared for blue/white screening?

X-gal (also known as 5-bromo-4-chloro-3-indolyl-?-D-galactoside) is soluble in either DMSO or DMF, and can be stored in solution in the freezer for up to 6 months. Protect the solution from light.

To add X-gal to previously made agar plates, pipette 40 ?l of a 40 mg/ml stock solution onto a 100mm pre-poured agar plate and spread evenly with a sterile spreader. Let dry 15 minutes and store plates away from light.

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

引用および参考文献 (1)

引用および参考文献

Abstract

Identification of endopeptidase genes from the genomic sequence of Lactobacillus helveticus CNRZ32 and the role of these genes in hydrolysis of model bitter peptides.

Authors:Sridhar VR, Hughes JE, Welker DL, Broadbent JR, Steele JL,

Journal:Appl Environ Microbiol

PubMed ID:15932998

Genes encoding three putative endopeptidases were identified from a draft-quality genome sequence of Lactobacillus helveticus CNRZ32 and designated pepO3, pepF, and pepE2. The ability of cell extracts from Escherichia coli DH5alpha derivatives expressing CNRZ32 endopeptidases PepE, PepE2, PepF, PepO, PepO2, and PepO3 to hydrolyze the model bitter peptides, beta-casein (beta-CN) ... More