UltraPure™ TBE Buffer, 10X

いいえ、2X Environmental Master Mix (EMM) は、TaqMan Universal PCR Master Mix (with またはwithout AmpErase UNG)と同じものではありません。 2X Environmental Master Mix は、確実な特異性と高感度で、ターゲットの細菌性病原体を検出できるように特別に開発された試薬です。 このMaster Mix中には、AmpliTaq Gold DNA Polymeraseを、さらに高純度に精製したAmpliTaq Gold DNA Polymerase, LD が採用されています。この新しい酵素は、残存する細菌由来のDNAを完全に除去するために、酵素調製の段階から、あらゆる手が施されています。

No. Autoclaving at temperatures that are too high or pressures that are too high can hydrolyze the agarose polymer and weaken it.

Add the powder to cold buffer while stirring. Let the agarose rehydrate for 1 to 2 hr and then heat slowly until agarose is completely dissolved. Using a microwave at low power settings is acceptable.

One recommendation: get a supply of fabric softener sheets and wipe the bottle with a sheet before opening it.

The precipitation of concentrated TBE stocks may be due to nucleation of salt crystals by dust particles or other insoluble materials. Therefore, filtering the solution through a 0.2 µm cellulose acetate or cellulose nitrate filter after preparation helps avoid this precipitate. [Mayeda A, Krainer A (1991) BioTechniques 10.2, 1820]