100 bp DNA Ladder

Citations & References (6)

Invitrogen™

100 bp DNA Ladder

Name: 100 bp DNA Ladder
SKU: 15628050

Invitrogen 100 bp DNAラダーは、100 bp～2,000 bpの範囲の二本鎖DNAのサイジングおよび近似定量用に設計されています。100 bp DNAラダーは詳細を見る

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製品番号（カタログ番号）	数量
15628050	500 μL DNAラダー、1 mL 10Xローディングバッファー
15628019	100 μL DNAラダー、1 mL 10Xサンプルローディングバッファー、100のアプリケーション

2 オプション

製品番号（カタログ番号） 15628050

価格（JPY）

101,500

Online offer

Ends: 26-Dec-2025

169,300

割引額 67,800 (40%)

Each

お問い合わせください ›

数量:

500 μL DNAラダー、1 mL 10Xローディングバッファー

一括またはカスタム形式をリクエストする

Invitrogen 100 bp DNAラダーは、100 bp～2,000 bpの範囲の二本鎖DNAのサイジングおよび近似定量用に設計されています。100 bp DNAラダーは、クロマトグラフィーで精製された13個のDNA断片で構成されており、2,000、1,500、および600 bpのリファレンスバンドを有しているため、簡単に配向できます。

100 bp DNAラダーは、1–2%アガロースゲルでの分離に最適です。

100 bp DNAラダーの特長：
•シャープで透明なバンド—クロマトグラフィー精製フラグメントにより、一貫性と信頼性の高い結果が得られます
• 便利—サンプルDNAの移動を追跡する10Xブルージュースゲルローディングバッファーも提供いたします
• 正確—各バンドには正確な量のDNAが含まれています

製品使用
二本鎖DNAラダーは、エチジウムブロマイドまたはSYBRセーフ染色後に1–2%アガロースゲルで可視化できます。ラダーは、均一な強度のDNAバンドで設計されており、各バンドを明確に確認できます。各バンド内の正確なDNA量により、DNAサンプルの近似定量が可能になります。

このラダーは、T4ポリヌクレオチドキナーゼまたはT4 DNAポリメラーゼを使用して放射活性標識できます。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

濃度0.5 μg/μL

ゲル適合性アガロースゲル

グリーン機能持続可能なパッケージ

キット内容500 μL DNA Ladder, 1 mL 10X Loading Buffer

反応数アプリケーション（x 500）

製品タイプDNAラダー

数量500 μL DNAラダー、1 mL 10Xローディングバッファー

ロード可能状態不可

サンプル充填量1 mL

出荷条件室温またはドライアイスでの出荷

技術クロマトグラフィーで精製された個々のDNA断片

容量（メートル法）500 µL

ゲルタイプアガロース

サイズ範囲100 bp～2,000 bp

Unit SizeEach

組成および保存条件

• 500 µL 100 bp DNAラダー
• 1 mL 10X BlueJuiceゲルローディングバッファー

-20℃で保管してください。

よくあるご質問（FAQ）

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?

Invitrogen DNA ladders contain linear dsDNA fragments.

Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?

Invitrogen DNA ladders are composed of double-stranded DNA fragments only.

Why are the DNA bands from my molecular weight ladder smearing?

Here are a few reasons why you might see smearing of the bands:

- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.

I'm seeing anomalous migration of my DNA ladder. What happened?

This can happen if the marker was heated. Please ensure that the ladders are not heated before use.

View all 100 bp DNA Ladder, 500 μL DNA Ladder, 1 mL 10X Loading Buffer FAQs

引用および参考文献 (6)

引用および参考文献

Abstract

A duplex PCR-based assay for measuring the amount of bacterial contamination in a nucleic acid extract from a culture of free-living protists.

Authors:Marron AO, Akam M, Walker G

Journal:PLoS One

PubMed ID:23593495

'Cultures of heterotrophic protists often require co-culturing with bacteria to act as a source of nutrition. Such cultures will contain varying levels of intrinsic bacterial contamination that can interfere with molecular research and cause problems with the collection of sufficient material for sequencing. Measuring the levels of bacterial contamination for ... More

Identification and characterization of non-saccharomyces spoilage yeasts isolated from Brazilian wines.

Authors:Echeverrigaray S, Randon M, da Silva K, Zacaria J, Delamare AP

Journal:World J Microbiol Biotechnol

PubMed ID:23355138

'The industry of fine wines and also locally consumed table wines is emerging in Brazil with an increasing volume and economic impact. Enologists in this region currently lack information about the prevalence and characteristics of spoilage yeasts, which may contaminate and potentially undervalue Brazilian wines. Herein, we analyzed 50 local ... More

Systemically administered gp100 encoding DNA vaccine for melanoma using water-in-oil-in-water multiple emulsion delivery systems.

Authors:Kalariya M, Amiji MM

Journal:

PubMed ID:23702000

'The purpose of this study was to develop a water-in-oil-in-water (W/O/W) multiple emulsions-based vaccine delivery system for plasmid DNA encoding the gp100 peptide antigen for melanoma immunotherapy. The gp100 encoding plasmid DNA was encapsulated in the inner-most aqueous phase of squalane oil containing W/O/W multiple emulsions using a two-step emulsification ... More

Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter.

Authors:Carter L, Lindsey LA, Grim CJ, Sathyamoorthy V, Jarvis KG, Gopinath G, Lee C, Sadowski JA, Trach L, Pava-Ripoll M, McCardell BA, Tall BD, Hu L

Journal:Appl Environ Microbiol

PubMed ID:23144142

In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates ... More

Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping of mycobacterium intracellulare for strain comparison with establishment of a PCR-based database.

Authors:Iakhiaeva E, McNulty S, Brown Elliott BA, Falkinham JO, Williams MD, Vasireddy R, Wilson RW, Turenne C, Wallace RJ

Journal:J Clin Microbiol

PubMed ID:23175249

Strain comparison is important to population genetics and to evaluate relapses in patients with Mycobacterium avium complex (MAC) lung disease, but the "gold standard" of pulsed-field gel electrophoresis (PFGE) is time-consuming and complex. We used variable-number tandem repeats (VNTR) for fingerprinting of respiratory isolates of M. intracellulare from patients with ... More

View all 100 bp DNA Ladder, 500 μL DNA Ladder, 1 mL 10X Loading Buffer citations