What species of salmon is Ultrapure Salmon Sperm DNA derived from?
The species of salmon is Oncerhyncus keta.
Is Ultrapure Salmon Sperm DNA double stranded? How can I make it single stranded?
Ultrapure Salmon Sperm DNA is double stranded. It can be made single stranded by heating to 95 degrees C and then cooling rapidly on ice. Cooling is usually done with the DNA diluted in a hybridization solution which keeps it single stranded because of the large dilution volume.
What concentration should the UltraPure Salmon Sperm DNA Solution (Cat. No. 15632011) be used at?
Carrier DNA is typically used at a concentration of 100 µg/mL in both the prehybridization and hybridization solutions.
引用および参考文献 (8)
引用および参考文献
Abstract
The human organic cation transporter (hOCT2) recognizes the degree of substrate ionization.
Authors: Barendt Wendy M; Wright Stephen H;
Journal:J Biol Chem
PubMed ID:11953440
'The organic cation transporter, OCT2, plays a role in renal secretion of a broad array of weak bases. To determine whether the degree of ionization of these compounds plays a role in their interaction with OCT2, we examined the influence of external pH values on the activity of the human ... More
The role of polymer nanolayer architecture on the separation performance of anion-exchange membrane adsorbers: part II. DNA and virus separations.
Authors:Bhut BV, Weaver J, Carter AR, Wickramasinghe SR, Husson SM,
Journal:Biotechnol Bioeng
PubMed ID:21618476
'The surface-initiated polymerization protocol developed in part I was used to prepare strong anion-exchange membranes with variable polymer chain graft densities and degrees of polymerization for DNA and virus particle separations. A focus of part II was to evaluate the role of polymer nanolayer architecture on DNA and virus binding. ... More
Spectrophotometric analysis of nucleic acids: oxygenation-dependent hyperchromism of DNA.
Authors:Doshi R, Day PJ, Carampin P, Blanch E, Stratford IJ, Tirelli N,
Journal:Anal Bioanal Chem
PubMed ID:20169336
'The absorbance at 260 nm (A(260)) is ubiquitously used for nucleic acid quantification. We show that following oxygenation, DNA solutions experience alterations in both spectral properties (hyperchromism in the UV region, lambda(max) 260 nm) and DNA conformation. The spectral changes caused by oxygen-DNA complexation are stable for at least several ... More
A quantitative real-time polymerase chain reaction assay for the seagrass pathogen Labyrinthula zosterae.
'The protist Labyrinthula zosterae (Phylum Bigyra, sensu Tsui et al. 2009) has been identified as a causative agent of wasting disease in eelgrass (Zostera marina), of which the most intense outbreak led to the destruction of 90% of eelgrass beds in eastern North America and western Europe in the 1930s. ... More
Noncoding DNA in lipofection of HeLa cells-a few insights.
Authors:Symens N, Rejman J, Lucas B, Demeester J, De Smedt SC, Remaut K,
Journal:Mol Pharm
PubMed ID:23421924
'In cationic carrier-mediated gene delivery, the disproportional relationship between the quantity of delivered DNA and the amount of encoded protein produced is a well-known phenomenon. The numerous intracellular barriers which need to be overcome by pDNA to reach the nucleoplasm play a major role in it. In contrast to what ... More