Gentamicin (10 mg/mL)

Citations & References (6)

Gibco™

Gentamicin (10 mg/mL)

Name: Gentamicin (10 mg/mL)
SKU: 15710064

ゲンタマイシン硫酸塩は、菌類Micromonospora Purpureaから精製された水溶性抗生物質薬物です。ゲンタマイシンは、細菌リボソームの30Sサブユニットに結合することで作用し、感受性細菌のタンパク質合成を阻害し、死滅させます。Gibco™ゲンタマイシンは、さまざまなグラム陽性細菌および一部のグラム陰性細菌に対して効果的で、細胞培養の細菌汚染防止のために使用されます詳細を見る

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製品番号（カタログ番号）	数量
15710064	10 mL
15710072	10 x 10 mL

2 オプション

製品番号（カタログ番号） 15710064

価格（JPY）

6,100

Each

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数量:

10 mL

ゲンタマイシン硫酸塩は、菌類Micromonospora Purpureaから精製された水溶性抗生物質薬物です。ゲンタマイシンは、細菌リボソームの30Sサブユニットに結合することで作用し、感受性細菌のタンパク質合成を阻害し、死滅させます。Gibco™ゲンタマイシンは、さまざまなグラム陽性細菌および一部のグラム陰性細菌に対して効果的で、細胞培養の細菌汚染防止のために使用されます。推奨される使用濃度範囲は0.5～50 µg/mlです。当社は、細胞培養アプリケーション用のさまざまな抗生物質および抗真菌剤を提供しています。

製品使用
研究目的のみに使用できます。動物またはヒトの診断もしくは治療には使用できません。

2施設cGMP製造
Gibco™ゲンタマイシンは、ニューヨーク州グランドアイランドにあるcGMP準拠施設で製造されています。この施設は医療機器製造業者としてFDAに登録されており、ISO 13485の認証を受けています。また、サプライチェーン継続性のため、当社のスコットランド施設で製造した同等ののGibco™ゲンタマイシン製品を提供しています（15710-049）。この施設は医療機器製造業者としてFDAに登録されており、ISO 13485の認証を受けています。

研究用途にのみご使用ください。診断目的には使用できません。

仕様

濃度10 mg/mL

培養タイプMammalian Cell Culture

使用対象（アプリケーション）Prevention of Cell Culture Contamination

数量10 mL

品質保持期間24 Months

出荷条件Room Temperature

形状Liquid

製品タイプAntibiotic

無菌性Sterile-filtered

Unit SizeEach

組成および保存条件

Storage conditions: 15-30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

If Gentamicin (10 mg/mL) is accidentally stored at 2-8 degrees C, would it affect the stability of the antibiotic?

No, storing Gentamicin solution for several days at 2-8 degrees C will not have any negative impact on its performance or stability. However, as Gentamicin solution has been shown to be stable at room temperature, the recommended storage temperature is ~25 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (6)

引用および参考文献

Abstract

Novel Cav2.1 splice variants isolated from Purkinje cells do not generate P-type Ca2+ current.

Authors: Tsunemi Taiji; Saegusa Hironao; Ishikawa Kinya; Nagayama Shin; Murakoshi Takayuki; Mizusawa Hidehiro; Tanabe Tsutomu;

Journal:J Biol Chem

PubMed ID:11756409

'The alpha(1)2.1 (alpha(1A)) subunits of P-type and Q-type Ca(2+) channels are encoded by a single gene, Cacna1a. Although these channels differ in the inactivation kinetics and sensitivity to omega-agatoxin IVA, the mechanism underlying these differences remains to be clarified. Alternative splicings of the Cacna1a transcript have been postulated to contribute ... More

Cell-autonomous impact of polysialic acid-producing enzyme ST8SIA2 on developmental migration and distribution of cortical interneurons.

Authors:Schuster UE, Rossdam C, Röckle I, Schiff M, Hildebrandt H

Journal:J Neurochem

PubMed ID:31608978

'In humans, variations in the polysialic acid-producing enzyme ST8SIA2 and disturbances in the cortical inhibitory system are associated with neurodevelopmental psychiatric disorders such as schizophrenia and autism. In mice, the ST8SIA2-dependent formation of polysialic acid during embryonic development is crucial for the establishment of interneuron populations of the medial prefrontal ... More

Identification of Novel Protein Targets of Dimethyl Fumarate Modification in Neurons and Astrocytes Reveals Actions Independent of Nrf2 Stabilization.

Authors:Piroli GG, Manuel AM, Patel T, Walla MD, Shi L, Lanci SA, Wang J, Galloway A, Ortinski PI, Smith DS, Frizzell N

Journal:Mol Cell Proteomics

PubMed ID:30587509

'The fumarate ester dimethyl fumarate (DMF) has been introduced recently as a treatment for relapsing remitting multiple sclerosis (RRMS), a chronic inflammatory condition that results in neuronal demyelination and axonal loss. DMF is known to act by depleting intracellular glutathione and modifying thiols on Keap1 protein, resulting in the stabilization ... More

Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.

Authors:Kueck T, Bloyet LM, Cassella E, Zang T, Schmidt F, Brusic V, Tekes G, Pornillos O, Whelan SPJ, Bieniasz PD

Journal:J Virol

PubMed ID:31578292

'Interferons (IFNs) induce the expression of interferon-stimulated genes (ISGs), many of which are responsible for the cellular antiviral state in which the replication of numerous viruses is blocked. How the majority of individual ISGs inhibit the replication of particular viruses is unknown. We conducted a loss-of-function screen to identify genes ... More

Use of Precision-Cut Lung Slices as an

Authors:Rosales Gerpe MC, van Vloten JP, Santry LA, de Jong J, Mould RC, Pelin A, Bell JC, Bridle BW, Wootton SK

Journal:Mol Ther Methods Clin Dev

PubMed ID:30112421

Organotypic slice cultures recapitulate many features of an intact organ, including cellular architecture, microenvironment, and polarity, making them an ideal tool for the

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