384 Well Black Plate, Coverglass Bottom, TC Surface, Pack of 6

Our Nunclon Delta surface for adherent cells is a standard tissue culture (TC) surface modification that makes the polystyrene surface more hydrophilic. This facilitates maximum adhesion for a broad range of cell types.

The Nunclon Delta surface is Thermo Scientific Nunclon Delta-certified, which is a Thermo Fisher proprietary certification that has a quality standard to help ensure consistency. Every lot of Thermo Scientific Nunclon Delta-certified cell cultureware is tested with Gibco media for:
- Less than 10% growth variation in L929, Hel299 and primary chick embryo cells
- Less than 15% variation colony formation of V79-4 cells

More information and all applicable products can be found in the "Product selection guide for every step of your cell culture workflow" brochure .

We offer Nunc Microplate Lids: Cat. No. 448143, that fit both the 384-well microplates and the 1,536-well microplates.

The cell culture-treated surfaces are very similar but the wells of the Nunc 384-Well Microplates are smaller than the wells of the Nunc 96-Well Microplate.
Culture recommendations for the Nunc 384-Well Microplates are:
- 15 - 50 µL media per well
- 0.5 - 2.0 x 10^4 cells per well

We recommend using the Nunc 384-Well Black Microplates for cell-based assays using fluorescent detection. Viability stains such as fluorogenic esterases can be used for proliferation, toxicity, and drug resistance or sensitivity assays. Many types of fluorochrome-conjugated antibodies are available for performing cell-based immunoassays. Fluorescent reporter genes are widely used for determining cell transfection.
We recommend using the Nunc 384-Well White Microplates for cell-based assays using luminescent detection. Viability stains such as luciferin/luciferase can be used for proliferation, toxicity, and drug resistance or sensitivity assays. Cell-based immunoassays can be performed using a HRP-conjugated antibody with a luminol-based substrate. Luciferase reporter genes are widely used for determining cell transfection.

We offer Nunc Black or White 384-Well microplates that are intended for use in conjunction with 384-well microplate luminometers and fluorometers that have excitation and detection systems located above the plate. Due to the opaque quality of these microplates, all detection systems must rely on either luminescent or fluorescent reagents, such as viability stains that report cell activity and membrane integrity.