I want to pour my own gels. Which agarose should I use?
Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.
引用および参考文献 (20)
引用および参考文献
Abstract
Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures.
Authors:Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M,
Journal:Blood
PubMed ID:19369228
'The concept of endothelial progenitor cells (EPCs) has attracted considerable interest in cardiovascular research, but despite a decade of research there are still no specific markers for EPCs and results from clinical trials remain controversial. Using liquid chromatography-tandem mass spectrometry, we analyzed the protein composition of microparticles (MPs) originating from ... More
A phantom for diffusion-weighted MRI (DW-MRI).
Authors:Lavdas I, Behan KC, Papadaki A, McRobbie DW, Aboagye EO,
Journal:J Magn Reson Imaging
PubMed ID:23576443
'PURPOSE: To develop tissue-equivalent diffusivity materials and build a spherical diffusion phantom which mimics the conditions typically found in biological tissues. Also, to assess the reproducibility of ADC measurements from a whole-body diffusion protocol. MATERIALS AND METHODS: Nickel-doped agarose/sucrose gels were manufactured and used to build a spherical diffusion phantom ... More
Determinants of nucleosome organization in primary human cells.
Authors:Valouev A, Johnson SM, Boyd SD, Smith CL, Fire AZ, Sidow A,
Journal:Nature
PubMed ID:21602827
'Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodelling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome. Analyses of chromatin landscape have uncovered a variety ... More
Controlling cell position in complex heterotypic 3D microtissues by tissue fusion.
Authors:Rago AP, Dean DM, Morgan JR,
Journal:Biotechnol Bioeng
PubMed ID:19012266
'Tissue fusion and cell sorting are processes fundamental to developmental biology with applications in tissue engineering. We have designed a fusion assay to investigate the factors governing the fusion of microtissues and the cell sorting that occurs after fusion. Normal human fibroblast (NHF) spheroids were self-assembled and cultured for 1, ... More
Host alternation of chikungunya virus increases fitness while restricting population diversity and adaptability to novel selective pressures.
Authors:Coffey LL, Vignuzzi M,
Journal:J Virol
PubMed ID:21047966
'The mechanisms by which RNA arboviruses, including chikungunya virus (CHIKV), evolve and maintain the ability to infect vertebrate and invertebrate hosts are poorly understood. To understand how host specificity shapes arbovirus populations, we studied CHIKV populations passaged alternately between invertebrate and vertebrate cells (invertebrate ? vertebrate) to simulate natural alternation ... More