UltraPure™ Agarose

いいえ、2X Environmental Master Mix (EMM) は、TaqMan Universal PCR Master Mix (with またはwithout AmpErase UNG)と同じものではありません。 2X Environmental Master Mix は、確実な特異性と高感度で、ターゲットの細菌性病原体を検出できるように特別に開発された試薬です。 このMaster Mix中には、AmpliTaq Gold DNA Polymeraseを、さらに高純度に精製したAmpliTaq Gold DNA Polymerase, LD が採用されています。この新しい酵素は、残存する細菌由来のDNAを完全に除去するために、酵素調製の段階から、あらゆる手が施されています。

Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.

Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.

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