I want to pour my own gels. Which agarose should I use?
Our UltraPure Agarose is standard melting-point agarose designed for routine separation and analysis of DNA and RNA fragments in the 500-23,000 nt range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. We also offer an UltraPure Low Melting Point Agarose, which is ideal for resolving DNA fragments from 10 to 1,000 bp with a low melting temperature of 65°C or less.
How can generation of replication competent adenoviruses be avoided when using your pSilencer adeno 1.0-CMV System?
Although there is only a very small chance of creating replication competent virus, steps should still be taken to avoid added risk. The virus is expanded in two rounds of amplification, and all secondary expansions should be performed from an initial expansion stock. Secondary amplifications in HEK293 cells should not be performed from the stock of another secondary expansion, as this could increase the chance of making replication competent virus. See the product manual for more detail and guidelines for screening.
Find additional tips, troubleshooting help, and resources within our RNAi Support Center.
引用および参考文献 (6)
引用および参考文献
Abstract
Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity.
'The purpose of this study was to investigate the neuroprotective effect of a water-soluble formulation of coenzyme Q10 (WS-CoQ10) in PC12 cells exposed to cisplatin, a chemotherapeutic agent with a dose-limiting factor due to neurotoxicity. In the cytokinesis-block micronucleus cytome assay (CBMN Cyt), WS-CoQ10 (at concentrations of 0.1, 0.5 and ... More
Spontaneous glycine-induced calcium transients in spinal cord progenitors promote neurogenesis.
Authors:Brustein E, Côté S, Ghislain J, Drapeau P
Journal:Dev Neurobiol
PubMed ID:22888055
Glycine and GABA are depolarizing during early development, but the purpose of this paradoxical chloride-mediated depolarization remains unclear, especially at early stages. It was previously reported that suppressing glycine signaling from the beginning of development in zebrafish embryos caused an abnormal maintenance of the progenitor population and a specific reduction ... More
Cytotoxicity and DNA fragmentation properties of butylated hydroxyanisole.
Authors:Vandghanooni S, Forouharmehr A, Eskandani M, Barzegari A, Kafil V, Kashanian S, Ezzati Nazhad Dolatabadi J
Journal:DNA Cell Biol
PubMed ID:23413972
Butylated hydroxyanisole (BHA) is a synthetic phenolic food additive that is used to prevent oils, fats, and shortenings from oxidative deterioration and rancidity. It was tested for potential cytotoxicity and genotoxicity upon A549 lung cancer cells. MTT assay and flow cytometry analysis were used for cytotoxicity assessment, whereas genotoxicity was ... More
A short cis-acting motif in the M112-113 promoter region is essential for IE3 to activate M112-113 gene expression and is important for murine cytomegalovirus replication.
Immediate-early 3 (IE3) gene products are required to activate early (E)-stage gene expression of murine cytomegaloviruses (MCMV). The first early gene activated by IE3 is the M112-113 gene (also called E1), although a complete understanding of the activation mechanism is still lacking. In this paper, we identify a 10-bp cis-regulating ... More
A mutant prion protein sensitizes neurons to glutamate-induced excitotoxicity.
Authors:Biasini E, Unterberger U, Solomon IH, Massignan T, Senatore A, Bian H, Voigtlaender T, Bowman FP, Bonetto V, Chiesa R, Luebke J, Toselli P, Harris DA
Journal:J Neurosci
PubMed ID:23392670
Growing evidence suggests that a physiological activity of the cellular prion protein (PrP(C)) plays a crucial role in several neurodegenerative disorders, including prion and Alzheimer's diseases. However, how the functional activity of PrP(C) is subverted to deliver neurotoxic signals remains uncertain. Transgenic (Tg) mice expressing PrP with a deletion of ... More