Collagenase, Type II, powder

製品番号（カタログ番号）	数量
17101015	1 g

製品番号（カタログ番号） 17101015

価格（JPY）

54,500

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コラゲナーゼは、コラーゲン内で高い頻度で検出されるPro-X-Glyc-Pro配列中の中性アミノ酸（X）とグリシン間の結合を切断するプロテアーゼです。コラゲナーゼは、皮膚、腱腱、血管、骨などの結合組織で一般的に見られる三重らせん天然コラーゲン原線維を分解できる点でプロテアーゼの中で特異的です。コラゲナーゼ分解は、ヒト腫瘍、マウス腎臓、ヒトの成人および胎児の脳、および上皮を含む多くの他の組織の培養に適しています。コラゲナーゼは比較的穏やかで、生理学的温度とpHで良好に解離し、機械的撹拌や特別な装置は必要ありません。

Gibco™コラゲナーゼII型は、ヒストリチクム菌から分離され、細胞または組織の解離および器官灌流における研究用の凍結乾燥非滅菌粉末としてパッケージされています。Gibco™コラゲナーゼII型の活性は、125ユニット／mgを超えることが保証されていますGibco™コラゲナーゼII型は、他のコラゲナーゼ製剤と比較して高いクロストリパイン活性を有し、心臓、骨、甲状腺、軟骨、および肝組織の消化に最適です。

研究用にのみ使用できます。診断用には使用いただけません。

数量1 g

品質保持期間24 Months

出荷条件Room Temperature

形状Lyophilized

製品タイプCollagenase

無菌性Non-sterile

Unit SizeEach

組成および保存条件

Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture

よくあるご質問（FAQ）

Can I reconstitute Collagenase, Type II, powder (Cat. No. 17101015) into DMEM or MEM alpha instead of HBSS with Ca and Mg?

Yes, Collagenase, Type II, powder can be reconstituted in DMEM or MEM alpha as long as the Mg and Ca concentrations in the medium are similar.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I make the 1000X stock (100 U/µL) solution from Collagenase powder?

1. Add 1 mL Hanks' Balanced Salt Solution (HBSS) with calcium and magnesium directly to 1 g vial of Collagenase. Vortex gently to ensure complete dissolution. Transfer to a clean tube.
2. Determine volume of HBSS (with calcium and magnesium) required to bring collagenase solution to 100 U/µL (1000X stock solution). The activity is lot- specific. Rinse vial with this volume of HBSS (with calcium and magnesium), and combine. Filter sterilize 1000X stock solution with a low protein binding filtration unit.
Example: Assuming the lot you have purchased has an activity of 265 U/mg, this lot will have 265000 Units per mL when you reconstitute collagenase into HBSS (with calcium and magnesium) at 1 g/mL. In order to dilute 265000 U/L to 100000 U/mL (= 100 U/µL), you need to dilute the 1 g/mL enzyme solution 2.65 fold.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

引用および参考文献 (2)

引用および参考文献

Abstract

Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts.

Authors:Sander V, Suñe G, Jopling C, Morera C, Izpisua Belmonte JC,

Journal:Nat Protoc

PubMed ID:23538883

'This protocol describes how to isolate primary cardiomyocytes from adult zebrafish hearts and culture them for up to 4 weeks, thereby using them as an alternative to in vivo experiments. After collagenase digestion of the ventricle, cells are exposed to increasing calcium concentrations in order to obtain high-purity cardiomyocytes. The ... More

The PDZ binding motif of the Beta 1 adrenergic receptor modulates receptor trafficking and signaling in cardiac myocytes.

Authors: Xiang Yang; Devic Eric; Kobilka Brian;

Journal:J Biol Chem

PubMed ID:12097326

'beta(1) and beta(2) adrenergic receptors (AR) regulate the intrinsic contraction rate in neonatal mouse cardiac myocytes through distinct signaling pathways. It has been shown that stimulation of beta(1)ARs leads to a protein kinase A-dependent increase in contraction rate. In contrast, stimulation of beta(2)ARs has a biphasic effect on contraction rate, ... More