Search
Citations & References (3)
Invitrogen™
Uracil DNA Glycosylase
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| 18054015 | 100 units |
製品番号（カタログ番号） 18054015
価格（JPY）お問い合わせください ›
23,200
Each
数量:
100 units
Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases. UDG will not remove uracil from RNA.
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 μg/ml 3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit of
enzyme in 50 μl for 10 min. at 37°C (4).
Applications: Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results (1,2) for cloning PCR fragments (3).
Source: Purified from E. coli expressing the E. coli ung gene on a plasmid (4,5).
Performance and Quality Testing: Exonuclease, endonuclease, and phosphatase assays.
Unit Definition: One unit catalyzes the release of 1 nmol of free uracil from 3H-poly(dU) in 1 h at 37°C.
Unit Reaction Conditions: 30 mM Tris-HCl (pH 8.3), 50 mM KCl, 5 mM MgCl2 , 4 μg/ml 3H-poly(dU)n at 7 x 10 5 cpm/μg, and 1 unit of
enzyme in 50 μl for 10 min. at 37°C (4).
研究用途にのみご使用ください。診断目的には使用できません。
仕様
ポリメラーゼDNA Polymerase
数量100 units
出荷条件Approved for shipment on Wet or Dry Ice
使用対象（アプリケーション）Standard PCR, Real Time PCR (qPCR)
形状液体
PCR法qPCR
Unit SizeEach
組成および保存条件
Store in freezer (-5° to -30°C).
よくあるご質問（FAQ）
What is UDG?
引用および参考文献 (3)
引用および参考文献
Abstract
A novel MHC class I-like gene is mutated in patients with hereditary haemochromatosis.
Journal:Nat Genet
PubMed ID:10545944
Hereditary haemochromatosis (HH), which affects some 1 in 400 and has an estimated carrier frequency of 1 in 10 individuals of Northern European descent, results in multi-organ dysfunction caused by increased iron deposition, and is treatable if detected early. Using linkage-disequilibrium and full haplotype analysis, we have identified a 250-kilobase
Structural determinants for HIV-1 integrase inhibition by beta-diketo acids.
Journal:J Biol Chem
PubMed ID:11805103
Among all the HIV-1 integrase inhibitors, the beta-diketo acids (DKAs) represent a major lead in anti-HIV-1 integrase drug design. These derivatives inhibit the integration reaction in vitro with a strong specificity for the 3'-end joining step. They are also antiviral and inhibit integration in vivo. The aim of the present
Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates.
Journal:J Biol Chem
PubMed ID:11960995
The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG). MutY is a base excision repair (BER) glycosylase that removes misincorporated adenine residues from OG:A mispairs, as well as G:A and C:A mispairs. We